PMID- 25709636
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150224
LR  - 20220317
IS  - 1680-6433 (Print)
IS  - 2008-2177 (Electronic)
IS  - 1680-6433 (Linking)
VI  - 12
IP  - 12
DP  - 2014 Dec
TI  - Melatonin improves development of early mouse embryos impaired by actinomycin-D 
      and TNF-alpha.
PG  - 799-804
AB  - BACKGROUND: Melatonin, a reactive oxygen species (ROS) scavenger and an 
      antioxidant, has been shown that can inhibit apoptosis. Administration of 
      melatonin may improve embryo development in assisted reproductive technology 
      (ART). OBJECTIVE: The aim of this study was to evaluate the role of melatonin in 
      inhibition of spontaneous and induced apoptosis by Tumor Necrosis Factor Alph 
      (TNF-alpha) and actinomycin-D during preimplantation development of mouse embryos. 
      MATERIALS AND METHODS: Female BALB/c mice were superovulated with pregnant mare 
      serum gonadotropin (PMSG) followed by human chorionic gonadotropin (HCG), then 
      allowed to mate with male mice. The resultant 2-cell embryos were divided into 
      six groups as follows: control (group I), melatonin (group II), actinomycin-D 
      (group III), actinomycin-D + melatonin (group IV), TNF-alpha (group V), and TNF-alpha + 
      melatonin (group VI). We recorded the numbers and developmental rates of the 
      4-cell, 8-cell, morula and blastocyst embryos. Blastocysts were stained with 
      acridine orange in order to assess for the embryo quality. RESULTS: The group IV 
      showed a significantly higher developmental rate of blastocysts compared to group 
      III (p<0.05). The number of dead blastomers was significantly decreased in group 
      IV in comparison to group III (p<0.05). Both V and VI groups had a lower 
      developmental rate and lesser quality of blastocysts compared with group I. There 
      was no significant difference in the developmental rate of blastocysts from group 
      II compared to group I (p<0.05). CONCLUSION: Supplementation of embryo culture 
      media with melatonin can improve the quality and developmental rate of embryos. 
      Melatonin can prevent cell death that was induced by TNF- alpha and actinomycine-D.
FAU - Niknafs, Behrooz
AU  - Niknafs B
AD  - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
FAU - Mehdipour, Ahmad
AU  - Mehdipour A
AD  - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
FAU - Mohammadi Roushandeh, Amaneh
AU  - Mohammadi Roushandeh A
AD  - Department of Anatomical Sciences, School of Medicine, Hamadan University of 
      Medical Sciences, Hamadan, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Reprod Med
JT  - Iranian journal of reproductive medicine
JID - 101463749
PMC - PMC4336669
OTO - NOTNLM
OT  - Actinomycine-D
OT  - Development
OT  - Embryo
OT  - Melatonin
OT  - TNF-alpha
EDAT- 2015/02/25 06:00
MHDA- 2015/02/25 06:01
PMCR- 2014/12/01
CRDT- 2015/02/25 06:00
PHST- 2014/02/15 00:00 [received]
PHST- 2014/06/11 00:00 [revised]
PHST- 2014/08/24 00:00 [accepted]
PHST- 2015/02/25 06:00 [entrez]
PHST- 2015/02/25 06:00 [pubmed]
PHST- 2015/02/25 06:01 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - ijrm-12-799 [pii]
PST - ppublish
SO  - Iran J Reprod Med. 2014 Dec;12(12):799-804.