PMID- 25710447
OWN - NLM
STAT- MEDLINE
DCOM- 20150702
LR  - 20181202
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 161
DP  - 2015 Apr
TI  - Development of a comet-FISH assay for the detection of DNA damage in hemocytes of 
      Crassostrea gigas.
PG  - 189-95
LID - S0166-445X(15)00030-2 [pii]
LID - 10.1016/j.aquatox.2015.01.022 [doi]
AB  - In this work, the DNA-damaging effect of hydrogen peroxide on the structural 
      integrity of nucleolar organizer regions (NORs) was studied for the first time by 
      comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was 
      assessed in hemocytes using an alkaline version of the comet assay. Next, NOR 
      sensitivity was analyzed by mapping major rDNA repeat unit by fluorescence in 
      situ hybridization (FISH) on the same comet slides. Exposure of hemocytes to 100 
      muM of hydrogen peroxide induced a significant increase in both DNA damage and 
      number of FISH-signals of major ribosomal genes versus the control. Moreover, a 
      significant positive correlation was shown between DNA damage as measured by the 
      comet assay (percentage of DNA in comet tail) and the number of signals present 
      in comet tails. This study demonstrates the potential value of the comet-FISH 
      assay for the study of DNA damage induced by genotoxicant exposure of target 
      genes. It offers a perspective for better understanding the impact of 
      genotoxicity on animal physiology and fitness.
CI  - Copyright (c) 2015. Published by Elsevier B.V.
FAU - Perez-Garcia, C
AU  - Perez-Garcia C
AD  - IFREMER, Department of Biogeochemistry and Ecotoxicology, Laboratory of 
      Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France. 
      Electronic address: mcperezg@ifremer.fr.
FAU - Rouxel, J
AU  - Rouxel J
AD  - IFREMER, Department of Biogeochemistry and Ecotoxicology, Laboratory of 
      Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France.
FAU - Akcha, F
AU  - Akcha F
AD  - IFREMER, Department of Biogeochemistry and Ecotoxicology, Laboratory of 
      Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France. 
      Electronic address: fakcha@ifremer.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150129
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Water Pollutants, Chemical)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - *Comet Assay
MH  - Crassostrea/*drug effects
MH  - DNA Damage/*drug effects
MH  - Hemocytes/drug effects
MH  - Hydrogen Peroxide/toxicity
MH  - *In Situ Hybridization, Fluorescence
MH  - Toxicity Tests/*methods
MH  - Water Pollutants, Chemical/toxicity
OTO - NOTNLM
OT  - Comet assay
OT  - Comet-FISH
OT  - Crassostrea gigas
OT  - Genotoxicity
OT  - Hydrogen peroxide
EDAT- 2015/02/25 06:00
MHDA- 2015/07/03 06:00
CRDT- 2015/02/25 06:00
PHST- 2014/07/24 00:00 [received]
PHST- 2015/01/14 00:00 [revised]
PHST- 2015/01/25 00:00 [accepted]
PHST- 2015/02/25 06:00 [entrez]
PHST- 2015/02/25 06:00 [pubmed]
PHST- 2015/07/03 06:00 [medline]
AID - S0166-445X(15)00030-2 [pii]
AID - 10.1016/j.aquatox.2015.01.022 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2015 Apr;161:189-95. doi: 10.1016/j.aquatox.2015.01.022. Epub 2015 
      Jan 29.