PMID- 25711740
OWN - NLM
STAT- MEDLINE
DCOM- 20160215
LR  - 20181113
IS  - 1559-0305 (Electronic)
IS  - 1073-6085 (Linking)
VI  - 57
IP  - 6
DP  - 2015 Jun
TI  - Adenovirus Dodecahedron, a VLP, Can be Purified by Size Exclusion Chromatography 
      Instead of Time-Consuming Sucrose Density Gradient Centrifugation.
PG  - 565-73
LID - 10.1007/s12033-015-9850-9 [doi]
AB  - Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve 
      pentameric penton base (Pb) proteins, responsible for adenovirus cell 
      penetration. It is generated spontaneously in the baculovirus system upon 
      expression of the Pb gene of adenovirus serotype 3. This particle shows 
      remarkable cell penetration ability with 2,00,000-3,00,000 Dd internalized into 
      one cell in culture, conceivably delivering several millions of foreign cargo 
      molecules to the target cell. We have used it in the past for delivery of small 
      drugs as well as a vaccination platform, in which Dd serves as a particulate 
      vaccine delivery system. Since development of new biomedicals depends strongly on 
      the cost of their expression and purification, we attempted, albeit 
      unsuccessfully, to obtain Dd expression in bacteria. We therefore retained its 
      expression in the baculovirus/insect cells system but introduced significant 
      improvements in the protocols for Dd expression and purification, leading to 
      considerable savings in time and improved yield.
FAU - Szurgot, I
AU  - Szurgot I
AD  - Institute of Biochemistry and Biophysics of Polish Academy of Sciences, 
      Pawinskiego 5a, 02106, Warsaw, Poland.
FAU - Jedynak, M
AU  - Jedynak M
FAU - Podsiadla-Bialoskorska, M
AU  - Podsiadla-Bialoskorska M
FAU - Piwowarski, Jan
AU  - Piwowarski J
FAU - Szolajska, E
AU  - Szolajska E
FAU - Chroboczek, J
AU  - Chroboczek J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (Viral Proteins)
RN  - 57-50-1 (Sucrose)
SB  - IM
MH  - Adenoviridae/genetics/*metabolism
MH  - Centrifugation/*methods
MH  - Chromatography, Gel/*methods
MH  - Cloning, Molecular
MH  - Escherichia coli/genetics
MH  - Genes, Viral
MH  - Sucrose
MH  - Viral Proteins/genetics/*isolation & purification/metabolism
EDAT- 2015/02/26 06:00
MHDA- 2016/02/16 06:00
CRDT- 2015/02/26 06:00
PHST- 2015/02/26 06:00 [entrez]
PHST- 2015/02/26 06:00 [pubmed]
PHST- 2016/02/16 06:00 [medline]
AID - 10.1007/s12033-015-9850-9 [doi]
PST - ppublish
SO  - Mol Biotechnol. 2015 Jun;57(6):565-73. doi: 10.1007/s12033-015-9850-9.