PMID- 25712385
OWN - NLM
STAT- MEDLINE
DCOM- 20160111
LR  - 20150413
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 18
DP  - 2015 May
TI  - Brain derived neurotrophic factor release from layer-by-layer coated agarose 
      nerve guidance scaffolds.
PG  - 128-31
LID - S1742-7061(15)00077-X [pii]
LID - 10.1016/j.actbio.2015.02.014 [doi]
AB  - Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived 
      neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat 
      central nervous system. The purpose of this work was to explore whether agarose 
      NGS coated with hydrogen-bonded layer-by-layer (HLbL) could provide an acellular 
      method of delivering prolonged and consistent dosages of active BDNF. Our results 
      show that HLbL-coated agarose NGS could release BDNF over 10days in consistent 
      dosages averaging 80.5+/-12.5(SD)ng/mL. Moreover, the BDNF released from HLbL was 
      confirmed active by in vitro cell proliferation assays. To our knowledge, this is 
      the first report demonstrating that HLbL assembled onto a hydrogel can provide 
      consistent, prolonged release of active BDNF in clinically relevant dosages.
CI  - Copyright (c) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Lynam, Daniel A
AU  - Lynam DA
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, East Lansing, MI 48824, United States.
FAU - Shahriari, Dena
AU  - Shahriari D
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, East Lansing, MI 48824, United States.
FAU - Wolf, Kayla J
AU  - Wolf KJ
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, East Lansing, MI 48824, United States.
FAU - Angart, Phillip A
AU  - Angart PA
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, East Lansing, MI 48824, United States.
FAU - Koffler, Jacob
AU  - Koffler J
AD  - Center for Neural Repair, University of California, San Diego, CA 92093, United 
      States.
FAU - Tuszynski, Mark H
AU  - Tuszynski MH
AD  - Center for Neural Repair, University of California, San Diego, CA 92093, United 
      States.
FAU - Chan, Christina
AU  - Chan C
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, East Lansing, MI 48824, United States.
FAU - Walton, Patrick
AU  - Walton P
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, East Lansing, MI 48824, United States.
FAU - Sakamoto, Jeffrey
AU  - Sakamoto J
AD  - Department of Chemical Engineering and Materials Science, Michigan State 
      University, East Lansing, MI 48824, United States.
LA  - eng
GR  - R01EB014986/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150221
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 9012-36-6 (Sepharose)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Coated Materials, Biocompatible/*pharmacology
MH  - *Guided Tissue Regeneration
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Nerve Regeneration/*drug effects
MH  - Rats
MH  - Receptor, trkB/metabolism
MH  - Sepharose/*chemistry
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - Agarose
OT  - BDNF
OT  - Controlled release
OT  - Hydrogel
OT  - Nerve
EDAT- 2015/02/26 06:00
MHDA- 2016/01/12 06:00
CRDT- 2015/02/26 06:00
PHST- 2014/08/25 00:00 [received]
PHST- 2015/01/31 00:00 [revised]
PHST- 2015/02/13 00:00 [accepted]
PHST- 2015/02/26 06:00 [entrez]
PHST- 2015/02/26 06:00 [pubmed]
PHST- 2016/01/12 06:00 [medline]
AID - S1742-7061(15)00077-X [pii]
AID - 10.1016/j.actbio.2015.02.014 [doi]
PST - ppublish
SO  - Acta Biomater. 2015 May;18:128-31. doi: 10.1016/j.actbio.2015.02.014. Epub 2015 
      Feb 21.