PMID- 25712854
OWN - NLM
STAT- MEDLINE
DCOM- 20160129
LR  - 20191210
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 44
IP  - 1
DP  - 2015 May
TI  - Resistance of Black-lip learl oyster, Pinctada margaritifera, to infection by 
      Ostreid herpes virus 1muvar under experimental challenge may be mediated by 
      humoral antiviral activity.
PG  - 232-40
LID - S1050-4648(15)00079-0 [pii]
LID - 10.1016/j.fsi.2015.02.026 [doi]
AB  - Ostreid herpesvirus 1 (OsHV-1) has induced mass mortalities of the larvae and 
      spat of Pacific oysters, Crassostrea gigas, in Europe and, more recently, in 
      Oceania. The production of pearls from the Black-lip pearl oyster, Pinctada 
      margaritifera, represents the second largest source of income to the economies of 
      French Polynesia and many Pacific Island nations that could be severely 
      compromised in the event of a disease outbreak. Coincidentally with the 
      occurrence of OsHV-1 in the southern hemisphere, C. gigas imported from New 
      Zealand and France into French Polynesia tested positive for OsHV-1. Although 
      interspecies viral transmission has been demonstrated, the transmissibility of 
      OsHV-1 to P. margaritifera is unknown. We investigated the susceptibility of 
      juvenile P. margaritifera to OsHV-1 muvar that were injected with tissue 
      homogenates sourced from either naturally infected or healthy C. gigas. The 
      infection challenge lasted 14 days post-injection (dpi) with sampling at 0, 1, 2, 
      3, 5, 7 and 14 days. Mortality rate, viral prevalence, and cellular immune 
      responses in experimental animals were determined. Tissues were screened by light 
      microscopy and TEM. Pacific oysters were also challenged and used as a positive 
      control to validate the efficiency of OsHV-1 muvar infection. Viral particles and 
      features such as marginated chromatin and highly electron dense nuclei were 
      observed in C. gigas but not in P. margaritifera. Mortality rates and hemocyte 
      immune parameters, including phagocytosis and respiratory burst, were similar 
      between challenged and control P. margaritifera. Herpesvirus-inhibiting activity 
      was demonstrated in the acellular fraction of the hemolymph from P. 
      margaritifera, suggesting that the humoral immunity is critical in the defence 
      against herpesvirus in pearl oysters. Overall, these results suggest that under 
      the conditions of the experimental challenge, P. margaritifera was not sensitive 
      to OsHV-1 muvar and was not an effective host/carrier. The nature and spectrum of 
      activity of the humoral antiviral activity is worthy of further investigation.
CI  - Crown Copyright (c) 2015. Published by Elsevier Ltd. All rights reserved.
FAU - Tan, Terence L S
AU  - Tan TL
AD  - The University of Queensland, School of Biological Sciences and Centre for Marine 
      Science, Brisbane, Queensland 4072, Australia.
FAU - Paul-Pont, Ika
AU  - Paul-Pont I
AD  - The University of Sydney, Faculty of Veterinary Science, Camden, New South Wales 
      2570, Australia.
FAU - Evans, Olivia M
AU  - Evans OM
AD  - The University of Sydney, Faculty of Veterinary Science, Camden, New South Wales 
      2570, Australia.
FAU - Watterson, Daniel
AU  - Watterson D
AD  - Australian Infectious Diseases Research Centre, School of Chemistry and Molecular 
      Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia.
FAU - Young, Paul
AU  - Young P
AD  - Australian Infectious Diseases Research Centre, School of Chemistry and Molecular 
      Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia.
FAU - Whittington, Richard
AU  - Whittington R
AD  - The University of Sydney, Faculty of Veterinary Science, Camden, New South Wales 
      2570, Australia.
FAU - Fougerouse, Angelique
AU  - Fougerouse A
AD  - Direction des Ressources Marines, Papeete, French Polynesia.
FAU - Bichet, Herve
AU  - Bichet H
AD  - Direction des Ressources Marines, Papeete, French Polynesia.
FAU - Barnes, Andrew C
AU  - Barnes AC
AD  - The University of Queensland, School of Biological Sciences and Centre for Marine 
      Science, Brisbane, Queensland 4072, Australia. Electronic address: 
      a.barnes@uq.edu.au.
FAU - Dang, Cecile
AU  - Dang C
AD  - The University of Queensland, School of Biological Sciences and Centre for Marine 
      Science, Brisbane, Queensland 4072, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150221
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
SB  - IM
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Disease Resistance/*immunology
MH  - Hemocytes/cytology/physiology
MH  - Herpesviridae/growth & development/*physiology
MH  - Immunity, Humoral
MH  - Phagocytosis
MH  - Pinctada/*immunology/*virology
MH  - Vero Cells
MH  - Viral Plaque Assay
OTO - NOTNLM
OT  - Hemocytes
OT  - Herpes
OT  - Humoral immunity
OT  - Ostreid herpesvirus
OT  - Pinctada
EDAT- 2015/02/26 06:00
MHDA- 2016/01/30 06:00
CRDT- 2015/02/26 06:00
PHST- 2014/06/06 00:00 [received]
PHST- 2015/01/19 00:00 [revised]
PHST- 2015/02/14 00:00 [accepted]
PHST- 2015/02/26 06:00 [entrez]
PHST- 2015/02/26 06:00 [pubmed]
PHST- 2016/01/30 06:00 [medline]
AID - S1050-4648(15)00079-0 [pii]
AID - 10.1016/j.fsi.2015.02.026 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2015 May;44(1):232-40. doi: 10.1016/j.fsi.2015.02.026. 
      Epub 2015 Feb 21.