PMID- 25713028
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20191210
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 29
IP  - 6
DP  - 2015 Jun
TI  - Neisseria meningitis GNA1030 is a ubiquinone-8 binding protein.
PG  - 2260-7
LID - 10.1096/fj.14-263954 [doi]
AB  - Bexsero, a new vaccine against Neisseria meningitidis serogroup B (MenB), is 
      composed of 3 main recombinant proteins and an outer membrane vesicle component. 
      One of the main bactericidal antigens, neisseria heparin binding antigen (NHBA), 
      is present as a fusion protein with the accessory protein genome-derived 
      neisserial antigen (GNA) 1030 to further increase its immunogenicity. The gene 
      encoding for GNA1030 is present and highly conserved in all Neisseria strains, 
      and although orthologs are present in numerous species, its biologic function is 
      unknown. Native mass spectrometry was used to demonstrate that GNA1030 forms a 
      homodimer associated with 2 molecules of ubiquinone-8 (Ub8), a cofactor mainly 
      involved in the electron transport chain and with antioxidant properties. Disc 
      diffusion assays on the wild-type and knockout mutant of GNA1030, in the presence 
      of various compounds, suggested that GNA1030 is not involved in oxidative stress 
      or electron chain transport per se, although it contributes to constitutive 
      refilling of the inner membrane with Ub8. These studies shed light on an 
      accessory protein present in Bexsero and reveal functional insights into the 
      family of related proteins. On the basis of our findings, we propose to name the 
      protein neisseria ubiquinone binding protein (NUbp).
CI  - (c) FASEB.
FAU - Donnarumma, Danilo
AU  - Donnarumma D
AD  - Novartis Vaccines, Siena, Italy.
FAU - Golfieri, Giacomo
AU  - Golfieri G
AD  - Novartis Vaccines, Siena, Italy.
FAU - Brier, Sebastien
AU  - Brier S
AD  - Novartis Vaccines, Siena, Italy.
FAU - Castagnini, Marta
AU  - Castagnini M
AD  - Novartis Vaccines, Siena, Italy.
FAU - Veggi, Daniele
AU  - Veggi D
AD  - Novartis Vaccines, Siena, Italy.
FAU - Bottomley, Matthew James
AU  - Bottomley MJ
AD  - Novartis Vaccines, Siena, Italy.
FAU - Delany, Isabel
AU  - Delany I
AD  - Novartis Vaccines, Siena, Italy.
FAU - Norais, Nathalie
AU  - Norais N
AD  - Novartis Vaccines, Siena, Italy nathalie.norais@novartis.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150220
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (4CMenB vaccine)
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Disulfides)
RN  - 0 (Meningococcal Vaccines)
RN  - 0 (Methacrylates)
RN  - 0 (Oxidants)
RN  - 0 (Periplasmic Proteins)
RN  - 0 (Thiazoles)
RN  - 1339-63-5 (Ubiquinone)
RN  - 642-15-9 (Antimycin A)
RN  - 6VY98BQ7NB (myxothiazol)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - CQA993F7P8 (ubiquinone 8)
RN  - EC 7.1.1.8 (Electron Transport Complex III)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anti-Bacterial Agents/pharmacology
MH  - Antigens, Bacterial/chemistry/genetics/*metabolism
MH  - Antimycin A/pharmacology
MH  - Bacterial Proteins/chemistry/genetics/*metabolism
MH  - Blotting, Western
MH  - Cell Survival/drug effects/genetics
MH  - Cloning, Molecular
MH  - Disulfides/metabolism
MH  - Electron Transport Complex III/antagonists & inhibitors/metabolism
MH  - Hydrogen Peroxide/pharmacology
MH  - Mass Spectrometry/methods
MH  - Meningococcal Vaccines/metabolism
MH  - Methacrylates/pharmacology
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Neisseria meningitidis/genetics/growth & development/*metabolism
MH  - Oxidants/pharmacology
MH  - Periplasmic Proteins/chemistry/genetics/metabolism
MH  - Protein Binding
MH  - Protein Multimerization
MH  - Thiazoles/pharmacology
MH  - Ubiquinone/*metabolism
OTO - NOTNLM
OT  - Bexsero vaccine
OT  - NUbp
OT  - native mass spectrometry
EDAT- 2015/02/26 06:00
MHDA- 2015/08/25 06:00
CRDT- 2015/02/26 06:00
PHST- 2014/10/21 00:00 [received]
PHST- 2015/01/20 00:00 [accepted]
PHST- 2015/02/26 06:00 [entrez]
PHST- 2015/02/26 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
AID - fj.14-263954 [pii]
AID - 10.1096/fj.14-263954 [doi]
PST - ppublish
SO  - FASEB J. 2015 Jun;29(6):2260-7. doi: 10.1096/fj.14-263954. Epub 2015 Feb 20.