PMID- 25713751
OWN - NLM
STAT- Publisher
LR  - 20191120
IS  - 2162-1918 (Print)
IS  - 2162-1934 (Electronic)
IS  - 2162-1918 (Linking)
VI  - 4
IP  - 2
DP  - 2015 Feb 1
TI  - Effects of High Estrogen Levels on Monocyte Chemoattractant Protein-1 and Wound 
      Healing.
PG  - 92-99
AB  - Objective: Herein, we tested the effects of high levels of supplemental estrogen 
      treatment on cutaneous wound healing. Approach: Female mice were implanted with a 
      17beta-estradiol (E(2)) secreting pellet or placebo before receiving a 
      full-thickness dermal excisional wound. Mice receiving the E(2) pellet attained 
      hormone levels that are comparable to those achieved during pregnancy. At 1, 3, 
      and 5 days after injury, the dermal excision wound was examined for their 
      histologic appearance, rate of closure, and chemokine levels. Results: Wound 
      closure, assessed by percent reepithelialization, was slower in E(2)-treated mice 
      relative to placebo (42.6%+/-6.6% vs. 70.0%+/-5.3%, respectively, 3 days after 
      injury). In addition, there was a marked reduction in the subepithelial 
      inflammatory infiltrate and granulation tissue in E(2)-treated mice relative to 
      placebo. Wound levels of monocyte chemoattractant protein-1 (MCP-1) were 
      increased by 3 days after injury and continued to rise at 5 days after injury in 
      placebo-treated mice (p<0.01). By contrast, MCP-1 levels were significantly 
      reduced at 3 and 5 days after injury in E(2)-treated mice relative to 
      placebo-treated controls (p<0.01). This attenuation could be reversed by 
      treatment with an estrogen receptor antagonist. Innovation: High levels of 
      estrogen are able to suppress normal wound closure. Conclusion: Dermal wound 
      healing can be altered by manipulating the gonadal steroid hormone levels. In 
      particular, high levels of estrogen can be utilized to slow down the rate of 
      wound healing through a reduction in the inflammatory response.
FAU - Plackett, Timothy P
AU  - Plackett TP
AD  - Department of Surgery, Loyola University Medical Center , Maywood, Illinois.
FAU - Gregory, Meredith S
AU  - Gregory MS
AD  - Schepens Eye Research Institute, Harvard University , Boston, Massachusetts.
FAU - Kovacs, Elizabeth J
AU  - Kovacs EJ
AD  - Department of Surgery, Loyola University Medical Center , Maywood, Illinois. ; 
      Burn and Shock Trauma Research Institute, Loyola University Medical Center , 
      Maywood, Illinois.
LA  - eng
GR  - R01 AG018859/AG/NIA NIH HHS/United States
GR  - R21 AI073987/AI/NIAID NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Adv Wound Care (New Rochelle)
JT  - Advances in wound care
JID - 101590593
PMC - PMC4321977
EDAT- 2015/02/26 06:00
MHDA- 2015/02/26 06:00
PMCR- 2015/02/01
CRDT- 2015/02/26 06:00
PHST- 2014/08/21 00:00 [received]
PHST- 2014/09/01 00:00 [accepted]
PHST- 2015/02/26 06:00 [entrez]
PHST- 2015/02/26 06:00 [pubmed]
PHST- 2015/02/26 06:00 [medline]
PHST- 2015/02/01 00:00 [pmc-release]
AID - 10.1089/wound.2014.0597 [pii]
AID - 10.1089/wound.2014.0597 [doi]
PST - ppublish
SO  - Adv Wound Care (New Rochelle). 2015 Feb 1;4(2):92-99. doi: 
      10.1089/wound.2014.0597.