PMID- 25714369
OWN - NLM
STAT- MEDLINE
DCOM- 20160118
LR  - 20221207
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 2
DP  - 2015
TI  - Pharmacokinetic study of 14-(3-methylbenzyl)matrine and 
      14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass 
      spectrometry.
PG  - e0116010
LID - 10.1371/journal.pone.0116010 [doi]
LID - e0116010
AB  - A rapid, sensitive and selective high-performance liquid chromatography-tandem 
      mass spectrometric method (HPLC-MS) was developed and validated to determine the 
      14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in 
      rat plasma in the present study. The analytes were separated using a C18 column 
      (1.9 mum, 2.1 mm x 100 mm) equipped with a Security Guard C18 column (5 mum, 2.1 mm 
      x 10 mm), followed by detection via triple-quadrupole mass spectrometry using an 
      electrospray ionization (ESI) source. Sample pretreatment involved one-step 
      protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and 
      pseudoephedrine hydrochloride was used as an internal standard. The method was 
      linear in the concentration range of 5-2000 ng/ml for both compounds. The 
      intra-day and inter-day relative standard deviations (RSDs) were less than 15%, 
      and all relative errors (REs) were within 15%. The proposed method enables the 
      unambiguous identification and quantification of these two compounds in vivo. 
      This study is the first to determine the 3MBM and 4MBM levels in rat plasma after 
      oral administration of these compounds. These results provide a meaningful basis 
      for evaluating the clinical applications of these medicines.
FAU - Jiang, Minjie
AU  - Jiang M
AD  - School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, 
      Nanning, 530004, China.
FAU - Wang, Lisheng
AU  - Wang L
AD  - School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, 
      Nanning, 530004, China.
FAU - Huang, Shulin
AU  - Huang S
AD  - School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China.
FAU - Xu, Liba
AU  - Xu L
AD  - School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China.
FAU - Hu, Chao
AU  - Hu C
AD  - School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China.
FAU - Jiang, Weizhe
AU  - Jiang W
AD  - School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150225
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Alkaloids)
RN  - 0 (Quinolizines)
RN  - 0 (Matrines)
SB  - IM
MH  - Alkaloids/administration & dosage/chemistry/*pharmacokinetics
MH  - Animals
MH  - *Chromatography, Liquid
MH  - Drug Stability
MH  - Male
MH  - Molecular Structure
MH  - Quinolizines/administration & dosage/chemistry/*pharmacokinetics
MH  - Rats
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - *Tandem Mass Spectrometry
MH  - Matrines
PMC - PMC4340794
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/02/26 06:00
MHDA- 2016/01/19 06:00
PMCR- 2015/02/25
CRDT- 2015/02/26 06:00
PHST- 2014/09/22 00:00 [received]
PHST- 2014/12/03 00:00 [accepted]
PHST- 2015/02/26 06:00 [entrez]
PHST- 2015/02/26 06:00 [pubmed]
PHST- 2016/01/19 06:00 [medline]
PHST- 2015/02/25 00:00 [pmc-release]
AID - PONE-D-14-42720 [pii]
AID - 10.1371/journal.pone.0116010 [doi]
PST - epublish
SO  - PLoS One. 2015 Feb 25;10(2):e0116010. doi: 10.1371/journal.pone.0116010. 
      eCollection 2015.