PMID- 25714670
OWN - NLM
STAT- MEDLINE
DCOM- 20150803
LR  - 20150502
IS  - 1522-1555 (Electronic)
IS  - 0193-1849 (Linking)
VI  - 308
IP  - 9
DP  - 2015 May 1
TI  - Enhanced fatty acid oxidation in adipocytes and macrophages reduces lipid-induced 
      triglyceride accumulation and inflammation.
PG  - E756-69
LID - 10.1152/ajpendo.00362.2014 [doi]
AB  - Lipid overload in obesity and type 2 diabetes is associated with adipocyte 
      dysfunction, inflammation, macrophage infiltration, and decreased fatty acid 
      oxidation (FAO). Here, we report that the expression of carnitine 
      palmitoyltransferase 1A (CPT1A), the rate-limiting enzyme in mitochondrial FAO, 
      is higher in human adipose tissue macrophages than in adipocytes and that it is 
      differentially expressed in visceral vs. subcutaneous adipose tissue in both an 
      obese and a type 2 diabetes cohort. These observations led us to further 
      investigate the potential role of CPT1A in adipocytes and macrophages. We 
      expressed CPT1AM, a permanently active mutant form of CPT1A, in 3T3-L1 CARDelta1 
      adipocytes and RAW 264.7 macrophages through adenoviral infection. Enhanced FAO 
      in palmitate-incubated adipocytes and macrophages reduced triglyceride content 
      and inflammation, improved insulin sensitivity in adipocytes, and reduced 
      endoplasmic reticulum stress and ROS damage in macrophages. We conclude that 
      increasing FAO in adipocytes and macrophages improves palmitate-induced 
      derangements. This indicates that enhancing FAO in metabolically relevant cells 
      such as adipocytes and macrophages may be a promising strategy for the treatment 
      of chronic inflammatory pathologies such as obesity and type 2 diabetes.
CI  - Copyright (c) 2015 the American Physiological Society.
FAU - Malandrino, Maria Ida
AU  - Malandrino MI
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Fucho, Raquel
AU  - Fucho R
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Weber, Mineia
AU  - Weber M
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Calderon-Dominguez, Maria
AU  - Calderon-Dominguez M
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Mir, Joan Francesc
AU  - Mir JF
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Valcarcel, Lorea
AU  - Valcarcel L
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Escote, Xavier
AU  - Escote X
AD  - Endocrinology and Diabetes Unit, Joan XXIII University Hospital, Institut 
      d'Investigacio Sanitaria Pere i Virgili, Universitat Rovira i Virgili, Tarragona, 
      Spain; Institut de Biomedicina de la Universitat de Barcelona de Diabetes y 
      Enfermedades Metabolicas Asociadas, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Gomez-Serrano, Maria
AU  - Gomez-Serrano M
AD  - Institut de Biomedicina de la Universitat de Barcelona Fisiopatologia de la 
      Obesidad y la Nutricion, Instituto de Salud Carlos III, Madrid, Spain; Instituto 
      de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones 
      Cientificas and Universidad Autonoma de Madrid, Madrid, Spain;
FAU - Peral, Belen
AU  - Peral B
AD  - Institut de Biomedicina de la Universitat de Barcelona Fisiopatologia de la 
      Obesidad y la Nutricion, Instituto de Salud Carlos III, Madrid, Spain; Instituto 
      de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones 
      Cientificas and Universidad Autonoma de Madrid, Madrid, Spain;
FAU - Salvado, Laia
AU  - Salvado L
AD  - Institut de Biomedicina de la Universitat de Barcelona de Diabetes y Enfermedades 
      Metabolicas Asociadas, Instituto de Salud Carlos III, Madrid, Spain; Pharmacology 
      Unit, Department of Pharmacology and Therapeutic Chemistry and Institut de 
      Biomedicina de la Universitat de Barcelona, Faculty of Pharmacy, University of 
      Barcelona, Barcelona, Spain; and.
FAU - Fernandez-Veledo, Sonia
AU  - Fernandez-Veledo S
AD  - Endocrinology and Diabetes Unit, Joan XXIII University Hospital, Institut 
      d'Investigacio Sanitaria Pere i Virgili, Universitat Rovira i Virgili, Tarragona, 
      Spain; Institut de Biomedicina de la Universitat de Barcelona de Diabetes y 
      Enfermedades Metabolicas Asociadas, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Casals, Nuria
AU  - Casals N
AD  - Institut de Biomedicina de la Universitat de Barcelona Fisiopatologia de la 
      Obesidad y la Nutricion, Instituto de Salud Carlos III, Madrid, Spain; Basic 
      Sciences Department, Faculty of Medicine and Health Sciences, Universitat 
      Internacional de Catalunya, Barcelona, Spain.
FAU - Vazquez-Carrera, Manuel
AU  - Vazquez-Carrera M
AD  - Institut de Biomedicina de la Universitat de Barcelona de Diabetes y Enfermedades 
      Metabolicas Asociadas, Instituto de Salud Carlos III, Madrid, Spain; Pharmacology 
      Unit, Department of Pharmacology and Therapeutic Chemistry and Institut de 
      Biomedicina de la Universitat de Barcelona, Faculty of Pharmacy, University of 
      Barcelona, Barcelona, Spain; and.
FAU - Villarroya, Francesc
AU  - Villarroya F
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Vendrell, Joan J
AU  - Vendrell JJ
AD  - Endocrinology and Diabetes Unit, Joan XXIII University Hospital, Institut 
      d'Investigacio Sanitaria Pere i Virgili, Universitat Rovira i Virgili, Tarragona, 
      Spain; Institut de Biomedicina de la Universitat de Barcelona de Diabetes y 
      Enfermedades Metabolicas Asociadas, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Serra, Dolors
AU  - Serra D
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain;
FAU - Herrero, Laura
AU  - Herrero L
AD  - Department of Biochemistry and Molecular Biology, Institut de Biomedicina de la 
      Universitat de Barcelona, Universitat de Barcelona, Barcelona, Spain; Institut de 
      Biomedicina de la Universitat de Barcelona Fisiopatologia de la Obesidad y la 
      Nutricion, Instituto de Salud Carlos III, Madrid, Spain; lherrero@ub.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150224
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Fatty Acids)
RN  - 0 (Lipids)
RN  - 0 (Triglycerides)
SB  - IM
EIN - Am J Physiol Endocrinol Metab. 2016 May 1;310(9):E795. PMID: 27140962
MH  - 3T3-L1 Cells
MH  - Adipocytes/*metabolism
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Cohort Studies
MH  - Diabetes Mellitus, Type 2/metabolism
MH  - Fatty Acids/*metabolism
MH  - Female
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Lipid Metabolism/*drug effects
MH  - Lipids/*pharmacology
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Obesity/metabolism
MH  - Oxidation-Reduction
MH  - Triglycerides/metabolism
OTO - NOTNLM
OT  - CPT1
OT  - adipocytes
OT  - fatty acid oxidation
OT  - inflammation
OT  - macrophages
OT  - obesity
OT  - type 2 diabetes
EDAT- 2015/02/26 06:00
MHDA- 2015/08/04 06:00
CRDT- 2015/02/26 06:00
PHST- 2014/07/30 00:00 [received]
PHST- 2015/02/19 00:00 [accepted]
PHST- 2015/02/26 06:00 [entrez]
PHST- 2015/02/26 06:00 [pubmed]
PHST- 2015/08/04 06:00 [medline]
AID - ajpendo.00362.2014 [pii]
AID - 10.1152/ajpendo.00362.2014 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2015 May 1;308(9):E756-69. doi: 
      10.1152/ajpendo.00362.2014. Epub 2015 Feb 24.