PMID- 25719492
OWN - NLM
STAT- MEDLINE
DCOM- 20160104
LR  - 20171116
IS  - 1557-8518 (Electronic)
IS  - 1540-4196 (Linking)
VI  - 13
IP  - 4
DP  - 2015 May
TI  - Potential involvement of nicotinamide N-methyltransferase in the pathogenesis of 
      metabolic syndrome.
PG  - 165-70
LID - 10.1089/met.2014.0134 [doi]
AB  - BACKGROUND: Metabolic syndrome is a complex disorder characterized by the 
      presence of insulin resistance (IR), type 2 diabetes mellitus (T2DM), impaired 
      glucose tolerance (IGT), or impaired fasting glucose (IFG), plus at least two of 
      the following conditions--hypertension, hyperlipidemia, obesity, and 
      microalbuminuria. Metabolic syndrome exposes patients to a greater risk of 
      developing cardiovascular disease (CVD) and is often associated with elevated 
      levels of homocysteine (Hcy). In the current work, we analyzed the expression of 
      nicotinamide N-methyltransferase (NNMT). Because NNMT is involved in Hcy 
      metabolism and participates in the regulation of the cellular and plasma levels 
      of this compound, we explored the role played by the enzyme in metabolic 
      syndrome. METHODS: Real-time PCR, immunohistochemistry, western blot analysis, 
      and catalytic activity assay were performed to evaluate NNMT expression levels in 
      adipose tissue from 10 Wistar Ottawa Karlsburg W (WOKW) rats, which are an animal 
      model for metabolic syndrome, and from 10 Dark Agouti (DA) rats as the 
      disease-resistant control strain. RESULTS: NNMT mRNA, protein, and activity 
      levels were significantly higher in adipose tissue obtained from WOKW rats 
      compared with those observed in adipose tissue of DA rats. CONCLUSION: Data 
      reported in this study represent the first evidence supporting the hypothesis 
      that NNMT could play an important role in the pathogenesis of metabolic syndrome 
      and could have a potential for the development of a targeted therapy.
FAU - Giuliante, Rachela
AU  - Giuliante R
AD  - 1 Department of Clinical Sciences, Polytechnic University of Marche , Ancona, 
      Italy .
FAU - Sartini, Davide
AU  - Sartini D
FAU - Bacchetti, Tiziana
AU  - Bacchetti T
FAU - Rocchetti, Romina
AU  - Rocchetti R
FAU - Kloting, Ingrid
AU  - Kloting I
FAU - Polidori, Carlo
AU  - Polidori C
FAU - Ferretti, Gianna
AU  - Ferretti G
FAU - Emanuelli, Monica
AU  - Emanuelli M
LA  - eng
PT  - Journal Article
DEP - 20150226
PL  - United States
TA  - Metab Syndr Relat Disord
JT  - Metabolic syndrome and related disorders
JID - 101150318
RN  - 0 (Blood Glucose)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Triglycerides)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 2.1.1.1 (Nicotinamide N-Methyltransferase)
SB  - IM
MH  - Adipose Tissue/enzymology
MH  - Animals
MH  - Blood Glucose
MH  - Cholesterol/blood
MH  - DNA, Complementary/biosynthesis
MH  - Female
MH  - Homocysteine/blood/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Metabolic Syndrome/*enzymology/pathology
MH  - Nicotinamide N-Methyltransferase/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Triglycerides/blood
EDAT- 2015/02/27 06:00
MHDA- 2016/01/05 06:00
CRDT- 2015/02/27 06:00
PHST- 2015/02/27 06:00 [entrez]
PHST- 2015/02/27 06:00 [pubmed]
PHST- 2016/01/05 06:00 [medline]
AID - 10.1089/met.2014.0134 [doi]
PST - ppublish
SO  - Metab Syndr Relat Disord. 2015 May;13(4):165-70. doi: 10.1089/met.2014.0134. Epub 
      2015 Feb 26.