PMID- 25719552
OWN - NLM
STAT- MEDLINE
DCOM- 20160118
LR  - 20220409
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 2
DP  - 2015
TI  - Low pH-induced changes of antioxidant enzyme and ATPase activities in the roots 
      of rice (Oryza sativa L.) seedlings.
PG  - e0116971
LID - 10.1371/journal.pone.0116971 [doi]
LID - e0116971
AB  - Soil acidification is the main problem in the current rice production. Here, the 
      effects of low pH on the root growth, reactive oxygen species metabolism, plasma 
      membrane functions, and the transcript levels of the related genes were 
      investigated in rice seedlings (Oryza sativa L.) in a hydroponic system at pH 
      3.5, 4.5, and 5.5. There were two hybrid rice cultivars in this trial, including 
      Yongyou 12 (YY12, a japonica hybrid) and Zhongzheyou 1 (ZZY1, an indica hybrid). 
      Higher H+ activity markedly decreased root length, the proportion of fine roots, 
      and dry matter production, but induced a significant accumulation of hydrogen 
      peroxide (H2O2), and led to serious lipid peroxidation in the roots of the two 
      varieties. The transcript levels of copper/zinc superoxide dismutase 1 (Cu/Zn 
      SOD1), copper/zinc superoxide dismutase 2 (Cu/Zn SOD2), catalase A (CATA) and 
      catalase B (CATB) genes in YY12 and ZZY1 roots were significantly down-regulated 
      after low pH exposure for two weeks. Meanwhile, a significant decrease was 
      observed in the expression of the P-type Ca2+-ATPases in roots at pH 3.5. The 
      activities of antioxidant enzymes (SOD, CAT) and plasma membrane (PM) Ca2+-ATPase 
      in the two varieties were dramatically inhibited by strong rhizosphere 
      acidification. However, the expression levels of ascorbate peroxidase 1 (APX1) 
      and PM H+-ATPase isoform 7 were up-regulated under H+ stress compared with the 
      control. Significantly higher activities of APX and PM H+-ATPase could contribute 
      to the adaptation of rice roots to low pH.
FAU - Zhang, Yi-Kai
AU  - Zhang YK
AD  - State Key Laboratory of Rice Biology, China National Rice Research Institute, 
      Hangzhou, Zhejiang, 310006, P. R. China.
FAU - Zhu, De-Feng
AU  - Zhu DF
AD  - State Key Laboratory of Rice Biology, China National Rice Research Institute, 
      Hangzhou, Zhejiang, 310006, P. R. China.
FAU - Zhang, Yu-Ping
AU  - Zhang YP
AD  - State Key Laboratory of Rice Biology, China National Rice Research Institute, 
      Hangzhou, Zhejiang, 310006, P. R. China.
FAU - Chen, Hui-Zhe
AU  - Chen HZ
AD  - State Key Laboratory of Rice Biology, China National Rice Research Institute, 
      Hangzhou, Zhejiang, 310006, P. R. China.
FAU - Xiang, Jing
AU  - Xiang J
AD  - State Key Laboratory of Rice Biology, China National Rice Research Institute, 
      Hangzhou, Zhejiang, 310006, P. R. China.
FAU - Lin, Xian-Qing
AU  - Lin XQ
AD  - State Key Laboratory of Rice Biology, China National Rice Research Institute, 
      Hangzhou, Zhejiang, 310006, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150226
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Soil)
RN  - EC 1.11.1.11 (Ascorbate Peroxidases)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
SB  - IM
MH  - Ascorbate Peroxidases/genetics/metabolism
MH  - Calcium-Transporting ATPases/genetics/*metabolism
MH  - Catalase/genetics/*metabolism
MH  - Chimera/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Oryza/*enzymology/genetics
MH  - Plant Proteins/genetics/metabolism
MH  - Plant Roots/enzymology
MH  - Proton-Translocating ATPases/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rhizosphere
MH  - Seedlings/enzymology
MH  - Soil/chemistry
MH  - Superoxide Dismutase/genetics/*metabolism
PMC - PMC4342341
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/02/27 06:00
MHDA- 2016/01/19 06:00
PMCR- 2015/02/26
CRDT- 2015/02/27 06:00
PHST- 2014/07/27 00:00 [received]
PHST- 2014/12/17 00:00 [accepted]
PHST- 2015/02/27 06:00 [entrez]
PHST- 2015/02/27 06:00 [pubmed]
PHST- 2016/01/19 06:00 [medline]
PHST- 2015/02/26 00:00 [pmc-release]
AID - PONE-D-14-33632 [pii]
AID - 10.1371/journal.pone.0116971 [doi]
PST - epublish
SO  - PLoS One. 2015 Feb 26;10(2):e0116971. doi: 10.1371/journal.pone.0116971. 
      eCollection 2015.