PMID- 25720470
OWN - NLM
STAT- MEDLINE
DCOM- 20151113
LR  - 20200407
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1282
DP  - 2015
TI  - The preparation of chicken tracheal organ cultures for virus isolation, 
      propagation, and titration.
PG  - 51-6
LID - 10.1007/978-1-4939-2438-7_5 [doi]
AB  - Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick 
      embryo trachea with beating cilia, have proved useful in the isolation of several 
      respiratory viruses and as a viral assay system, using ciliostasis as the 
      criterion for infection. A simple technique for the preparation of chicken 
      tracheal organ cultures in glass test tubes, in which virus growth and 
      ciliostasis can be readily observed, is described.
FAU - Hennion, Ruth M
AU  - Hennion RM
AD  - The Pirbright Institute, High Street, Compton, Newbury, Berkshire, RG20 7NN, UK, 
      ruth.hennion@pirbright.ac.uk.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Coronavirus/*physiology
MH  - Tissue Culture Techniques
MH  - Titrimetry
MH  - Trachea/virology
MH  - Viral Load
MH  - Virus Cultivation/methods
MH  - Virus Replication
PMC - PMC7122565
EDAT- 2015/02/28 06:00
MHDA- 2015/11/14 06:00
PMCR- 2020/04/03
CRDT- 2015/02/28 06:00
PHST- 2015/02/28 06:00 [entrez]
PHST- 2015/02/28 06:00 [pubmed]
PHST- 2015/11/14 06:00 [medline]
PHST- 2020/04/03 00:00 [pmc-release]
AID - 5 [pii]
AID - 10.1007/978-1-4939-2438-7_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2015;1282:51-6. doi: 10.1007/978-1-4939-2438-7_5.