PMID- 25721668
OWN - NLM
STAT- MEDLINE
DCOM- 20150625
LR  - 20211203
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 459
IP  - 1
DP  - 2015 Mar 27
TI  - Protein arginine methyltransferase 5 (PRMT5) is a novel coactivator of 
      constitutive androstane receptor (CAR).
PG  - 143-7
LID - S0006-291X(15)00313-7 [pii]
LID - 10.1016/j.bbrc.2015.02.085 [doi]
AB  - The constitutive androstane receptor (CAR) plays a key role in the expression of 
      xenobiotic/steroid and drug metabolizing enzymes and their transporters. In this 
      study, we demonstrated that protein arginine methyltransferase 5 (PRMT5) is a 
      novel CAR-interacting protein. Furthermore, the PRMT-dependent induction of a CAR 
      reporter gene, which was independent of methyltransferase activity, was enhanced 
      in the presence of steroid receptor coactivator 1 (SRC1), peroxisome 
      proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1alpha) or DEAD box 
      DNA/RNA helicase DP97. Using tetracycline inducible-hCAR system in HepG2 cells, 
      we showed that knockdown of PRMT5 with small interfering RNA suppressed 
      tetracycline -induced mRNA expression of CYP2B6 but not of CYP2C9 or CYP3A4. 
      PRMT5 enhanced phenobarbital-mediated transactivation of a 
      phenobarbital-responsive enhancer module (PBREM)-driven reporter gene in 
      co-operation with PGC-1alpha in rat primary hepatocytes. Based on these findings, we 
      suggest PRMT5 to be a gene (or promoter)-selective coactivator of CAR by 
      mediating the formation of complexes between hCAR and appropriate coactivators.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Kanno, Yuichiro
AU  - Kanno Y
AD  - Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, 
      Chiba 274-8510, Japan. Electronic address: ykanno@phar.toho-u.ac.jp.
FAU - Inajima, Jun
AU  - Inajima J
AD  - Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, 
      Chiba 274-8510, Japan.
FAU - Kato, Sayaka
AU  - Kato S
AD  - Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, 
      Chiba 274-8510, Japan.
FAU - Matsumoto, Maika
AU  - Matsumoto M
AD  - Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, 
      Chiba 274-8510, Japan.
FAU - Tokumoto, Chikako
AU  - Tokumoto C
AD  - Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, 
      Chiba 274-8510, Japan.
FAU - Kure, Yuki
AU  - Kure Y
AD  - Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, 
      Chiba 274-8510, Japan.
FAU - Inouye, Yoshio
AU  - Inouye Y
AD  - Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, 
      Chiba 274-8510, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150223
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Constitutive Androstane Receptor)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (PPARGC1A protein, human)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Transcription Factors)
RN  - EC 1.14.13.- (CYP2C9 protein, human)
RN  - EC 1.14.13.- (Cytochrome P-450 CYP2C9)
RN  - EC 1.14.14.1 (CYP2B6 protein, human)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP2B6)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP3A)
RN  - EC 1.14.14.55 (CYP3A4 protein, human)
RN  - EC 2.1.1.319 (PRMT5 protein, human)
RN  - EC 2.1.1.319 (Protein-Arginine N-Methyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 2.7.7.- (DDX54 protein, human)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Cell Line
MH  - Constitutive Androstane Receptor
MH  - Cytochrome P-450 CYP2B6/genetics/metabolism
MH  - Cytochrome P-450 CYP2C9/genetics/metabolism
MH  - Cytochrome P-450 CYP3A/genetics/metabolism
MH  - DEAD-box RNA Helicases/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Neoplasm Proteins/genetics/metabolism
MH  - Nuclear Receptor Coactivator 1/genetics/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - Protein-Arginine N-Methyltransferases/genetics/*metabolism
MH  - Receptors, Cytoplasmic and Nuclear/genetics/*metabolism
MH  - Transcription Factors/genetics/metabolism
MH  - Transcriptional Activation
OTO - NOTNLM
OT  - CAR
OT  - CYP2B6
OT  - CYP3A4
OT  - Nuclear receptor
OT  - PRMT5
EDAT- 2015/02/28 06:00
MHDA- 2015/06/26 06:00
CRDT- 2015/02/28 06:00
PHST- 2015/02/10 00:00 [received]
PHST- 2015/02/13 00:00 [accepted]
PHST- 2015/02/28 06:00 [entrez]
PHST- 2015/02/28 06:00 [pubmed]
PHST- 2015/06/26 06:00 [medline]
AID - S0006-291X(15)00313-7 [pii]
AID - 10.1016/j.bbrc.2015.02.085 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Mar 27;459(1):143-7. doi: 
      10.1016/j.bbrc.2015.02.085. Epub 2015 Feb 23.