PMID- 25721805
OWN - NLM
STAT- MEDLINE
DCOM- 20160115
LR  - 20150414
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 165
DP  - 2015 May 13
TI  - Gamisasangja-tang suppresses pruritus and atopic skin inflammation in the NC/Nga 
      murine model of atopic dermatitis.
PG  - 54-60
LID - S0378-8741(15)00119-1 [pii]
LID - 10.1016/j.jep.2015.02.040 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Gamisasangja-tang (GST) is a traditional herbal 
      formula prescribed for patients with intractable pruritus in association with 
      various inflammatory skin diseases. To evaluate the effects of GST on pruritic 
      skin inflammation and investigate its cellular and molecular mechanisms. 
      MATERIALS AND METHODS: We orally administered GST to NC/Nga (NC) mice, an animal 
      model of atopic dermatitis. Scratching frequency and the dermatitis index were 
      evaluated, and histological examination was performed using hematoxylin and eosin 
      and toluidine blue staining. The levels of interleukin (IL)-31 and T-helper cell 
      type 2 (TH2) cytokines were determined in both the dorsal skin and cultured 
      splenocytes by real-time polymerase chain reaction (PCR) and enzyme-linked 
      immunosorbent assay (ELISA), respectively. The serum levels of chemokines and 
      immunoglobulin E (IgE) were determined by ELISA. Changes in the inflammatory cell 
      population were analyzed by a hemocytometer. RESULTS: GST significantly lowered 
      scratching frequency and inhibited increases in dermatitis index, thickness of 
      epidermis/dermis and infiltration of chemokine (C-C motif) receptor 3 (CCR3)(+) 
      and cluster of differentiation (CD)117(+)/FcepsilonRIalpha (Fc fragment of IgE, high 
      affinity I, receptor for; alpha polypeptide)(+) cells in atopic skin. Both IL-31 
      mRNA expression and production were significantly reduced by GST, which was 
      accomrease in the levels of IL-4, IL-5, and IL-13. Further, GST treatment 
      suppressed the secretion of eotaxin, TARC (thymus and activation-regulated 
      chemokine), IgE, and increases in the number of basophils and eosinophils in the 
      blood. CONCLUSION: GST may have potential as an effective treatment for pruritic 
      skin disease such as atopic dermatitis.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Park, Bo-Kyung
AU  - Park BK
AD  - Laboratory of Pharmacology, College of Korean Medicine, Daejeon University, 
      Daejeon 300-706, Republic of Korea.
FAU - Park, Yang-Chun
AU  - Park YC
AD  - Department of Internal Medicine, Daejeon Korean Medicine Hospital of Daejeon 
      University, Daejeon 301-724, Republic of Korea.
FAU - Jung, In Chul
AU  - Jung IC
AD  - Department of Neuropsychiatry, Dunsan Korean Medicine Hospital of Daejeon 
      University, Daejeon 302-122, Republic of Korea.
FAU - Kim, Seung-Hyung
AU  - Kim SH
AD  - Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 
      300-706, Republic of Korea.
FAU - Choi, Jeong June
AU  - Choi JJ
AD  - Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 
      300-706, Republic of Korea.
FAU - Do, Moonho
AU  - Do M
AD  - College of Pharmacy, Gachon University, Incheon 406-799, Republic of Korea.
FAU - Kim, Sun Yeou
AU  - Kim SY
AD  - College of Pharmacy, Gachon University, Incheon 406-799, Republic of Korea.
FAU - Jin, Mirim
AU  - Jin M
AD  - Laboratory of Pharmacology, College of Korean Medicine, Daejeon University, 
      Daejeon 300-706, Republic of Korea. Electronic address: mirimj@dju.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150223
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Cytokines)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukins)
RN  - 0 (interleukin-31, mouse)
SB  - IM
MH  - Animals
MH  - Cytokines/analysis
MH  - Dermatitis, Atopic/*drug therapy/pathology
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Interleukins/analysis
MH  - Male
MH  - Mice
MH  - Pruritus/*drug therapy
MH  - Real-Time Polymerase Chain Reaction
MH  - Skin/chemistry/*drug effects/pathology
OTO - NOTNLM
OT  - Atopic skin inflammation
OT  - Chlorogenic acid (PubChem CID: 1794427)
OT  - Decursin (PubChem CID: 442126)
OT  - Gamisasangja-tang (GST)
OT  - IL-31
OT  - NC/Nga murine model
OT  - Pruritus
OT  - Torilin (PubChem CID:6450226)
OT  - Vitextin (PubChem CID: 5280441)
EDAT- 2015/02/28 06:00
MHDA- 2016/01/16 06:00
CRDT- 2015/02/28 06:00
PHST- 2014/10/28 00:00 [received]
PHST- 2015/01/30 00:00 [revised]
PHST- 2015/02/13 00:00 [accepted]
PHST- 2015/02/28 06:00 [entrez]
PHST- 2015/02/28 06:00 [pubmed]
PHST- 2016/01/16 06:00 [medline]
AID - S0378-8741(15)00119-1 [pii]
AID - 10.1016/j.jep.2015.02.040 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2015 May 13;165:54-60. doi: 10.1016/j.jep.2015.02.040. Epub 
      2015 Feb 23.