PMID- 25722208
OWN - NLM
STAT- MEDLINE
DCOM- 20150825
LR  - 20220310
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 56
IP  - 3
DP  - 2015 Feb 26
TI  - BDNF Rescues RGCs But Not Intrinsically Photosensitive RGCs in Ocular 
      Hypertensive Albino Rat Retinas.
PG  - 1924-36
LID - 10.1167/iovs.15-16454 [doi]
AB  - PURPOSE: To study the responses of the general population of retinal ganglion 
      cells (Brn3a(+)RGCs) versus the intrinsically photosensitive RGCs 
      (melanopsin-expressing RGCs [m(+)RGCs]) to ocular hypertension (OHT), the effects 
      of brain-derived neurotrophic factor (BDNF) on the survival of axonally intact 
      and axonally nonintact RGCs, and the correlation of vascular integrity with 
      sectorial RGC loss. METHODS: In Sprague-Dawley rats, 5 mug BDNF or vehicle was 
      intravitreally injected into the left eye followed by laser photocoagulation of 
      the limbal tissues. To identify RGCs with an active retrograde axonal transport, 
      Fluorogold was applied to both superior colliculi 1 week before euthanasia 
      (FG(+)RGCs). Retinas were dissected 12 or 15 days after lasering and 
      immunoreacted against Brn3a (to identify all RGCs except m(+)RGCs), melanopsin, 
      or RECA1 (inner retinal vasculature). RESULTS: Ocular hypertension resulted at 12 
      to 15 days in sectorial loss of FG(+)RGCs (78%-84%, respectively) while 
      Brn3a(+)RGCs were significantly greater, indicating that a substantial proportion 
      (approximately 21%-26%) of RGCs with their retrograde axonal transport impaired 
      survive in the retina. Brain-derived neurotrophic factor increased the survival 
      of Brn3a(+)RGCs to 81% to 67% at 12 to 15 days, respectively. The inner retinal 
      vasculature showed no abnormalities that could account for the sectorial loss of 
      RGCs. At 12 to 15 days, m(+)RGCs decreased to approximately 50% to 51%, but this 
      loss was diffuse across the retina and was not prevented by BDNF. CONCLUSIONS: 
      The responses of m(+)RGCs against OHT-induced retinal degeneration and 
      neuroprotection differ from those of Brn3a(+)RGCs; while OHT induces similar loss 
      of Brn3a(+)RGCs and m(+)RGCs, Brn3a(+)RGCs are lost in sectors and can be rescued 
      with BDNF, but m(+)RGCs do not respond to BDNF and their loss is diffuse.
CI  - Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.
FAU - Valiente-Soriano, Francisco J
AU  - Valiente-Soriano FJ
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, and 
      Instituto Murciano de Investigacion Biosanitaria Virgen de la Arrixaca 
      (IMIB-Arrixaca), Murcia, Spain.
FAU - Nadal-Nicolas, Francisco M
AU  - Nadal-Nicolas FM
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, and 
      Instituto Murciano de Investigacion Biosanitaria Virgen de la Arrixaca 
      (IMIB-Arrixaca), Murcia, Spain.
FAU - Salinas-Navarro, Manuel
AU  - Salinas-Navarro M
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, and 
      Instituto Murciano de Investigacion Biosanitaria Virgen de la Arrixaca 
      (IMIB-Arrixaca), Murcia, Spain.
FAU - Jimenez-Lopez, Manuel
AU  - Jimenez-Lopez M
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, and 
      Instituto Murciano de Investigacion Biosanitaria Virgen de la Arrixaca 
      (IMIB-Arrixaca), Murcia, Spain.
FAU - Bernal-Garro, Jose M
AU  - Bernal-Garro JM
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, and 
      Instituto Murciano de Investigacion Biosanitaria Virgen de la Arrixaca 
      (IMIB-Arrixaca), Murcia, Spain.
FAU - Villegas-Perez, Maria P
AU  - Villegas-Perez MP
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, and 
      Instituto Murciano de Investigacion Biosanitaria Virgen de la Arrixaca 
      (IMIB-Arrixaca), Murcia, Spain.
FAU - Agudo-Barriuso, Marta
AU  - Agudo-Barriuso M
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, and 
      Instituto Murciano de Investigacion Biosanitaria Virgen de la Arrixaca 
      (IMIB-Arrixaca), Murcia, Spain.
FAU - Vidal-Sanz, Manuel
AU  - Vidal-Sanz M
AD  - Departamento de Oftalmologia, Facultad de Medicina, Universidad de Murcia, and 
      Instituto Murciano de Investigacion Biosanitaria Virgen de la Arrixaca 
      (IMIB-Arrixaca), Murcia, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150226
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Rod Opsins)
RN  - 0 (Transcription Factor Brn-3A)
RN  - 0 (melanopsin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Death/drug effects
MH  - Disease Models, Animal
MH  - Female
MH  - Intraocular Pressure/drug effects
MH  - Intravitreal Injections
MH  - Lasers/adverse effects
MH  - Ocular Hypertension/*drug therapy/physiopathology
MH  - Photoreceptor Cells, Vertebrate/*drug effects/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/*injuries
MH  - Retinal Ganglion Cells/*drug effects/metabolism/pathology
MH  - Rod Opsins/metabolism
MH  - Transcription Factor Brn-3A/metabolism
OTO - NOTNLM
OT  - BDNF neuroprotection
OT  - Brn3a
OT  - Fluorogold
OT  - adult albino rats
OT  - axonal transport
OT  - experimental glaucoma
OT  - intrinsically photosensitive RGCs
OT  - laser-induced ocular hypertension
OT  - melanopsin
OT  - melanopsin retinal ganglion cells
OT  - ocular hypertension
OT  - spatial distribution
EDAT- 2015/02/28 06:00
MHDA- 2015/08/26 06:00
CRDT- 2015/02/28 06:00
PHST- 2015/02/28 06:00 [entrez]
PHST- 2015/02/28 06:00 [pubmed]
PHST- 2015/08/26 06:00 [medline]
AID - iovs.15-16454 [pii]
AID - 10.1167/iovs.15-16454 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2015 Feb 26;56(3):1924-36. doi: 10.1167/iovs.15-16454.