PMID- 25723663
OWN - NLM
STAT- MEDLINE
DCOM- 20151120
LR  - 20181202
IS  - 1536-3732 (Electronic)
IS  - 1049-2275 (Linking)
VI  - 26
IP  - 2
DP  - 2015 Mar
TI  - Mesenchymal stem cells secrete brain-derived neurotrophic factor and promote 
      retinal ganglion cell survival after traumatic optic neuropathy.
PG  - 548-52
LID - 10.1097/SCS.0000000000001348 [doi]
AB  - OBJECTIVE: The study aimed to investigate whether intravitreal injection of 
      mesenchymal stem cells (MSCs) cultivated in vitro could increase the number of 
      survived retinal ganglion cells (RGCs) after traumatic optic neuropathy and 
      sought to identify potential mechanisms underlying such growth. METHODS: The 
      right eye of 24 cats in the MSC transplantation group accepted intravitreal 
      injection of MSCs, and the other 24 cats in the phosphate buffer (PBS) control 
      group received isotonic saline after traumatic optic neuropathy. The RGCs' 
      survival rate in separated retinal and brain-derived neurotrophic factor (BDNF) 
      expression were observed by Dil labeling and Enzyme-Linked Immuno Sorbent Assay 
      (ELISA), respectively, at 3, 7, 14, and 28 days after transplantation. RESULTS: 
      Quantitative analysis showed that RGCs were significantly attenuated at 3, 7, 14, 
      and 28 days after transplantation in both areas of the retina (peripheral, P7d = 
      0.0011, P14d < 0.001, P28d < 0.001; central, P3d = 0.0437, P7d = 0.0067, P14d < 
      0.001, P28d < 0.001). Mean density of RGCs in the MSC transplantation group was 
      significantly higher than that of the PBS control group after 14 days of 
      treatment (P < 0.001). The homogenates BDNF (hBDNF) in the MSC transplantation 
      group was obviously higher than that in the PBS control group at 14 and 28 days 
      (P < 0.05). CONCLUSIONS: The MSCs transplanted into the retina of cats can slow 
      down RGC apoptosis and steadily express BDNF. The MSC-mediated neuroprotection 
      after optic nerve injury may be related to BNDF.
FAU - Junyi, Li
AU  - Junyi L
AD  - From the Department of Otolaryngology-Head and Neck Surgery, The Affiliated 
      Hospital of Medical College, Qingdao University, Qingdao, Shangdong, China.
FAU - Na, Li
AU  - Na L
FAU - Yan, Jiang
AU  - Yan J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Craniofac Surg
JT  - The Journal of craniofacial surgery
JID - 9010410
RN  - 0 (Brain-Derived Neurotrophic Factor)
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cats
MH  - Cell Survival/*physiology
MH  - Female
MH  - Intravitreal Injections
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Optic Nerve Injuries/*physiopathology
MH  - Rats, Sprague-Dawley
MH  - Retinal Ganglion Cells/*physiology
EDAT- 2015/02/28 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/02/28 06:00
PHST- 2015/02/28 06:00 [entrez]
PHST- 2015/02/28 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.1097/SCS.0000000000001348 [doi]
PST - ppublish
SO  - J Craniofac Surg. 2015 Mar;26(2):548-52. doi: 10.1097/SCS.0000000000001348.