PMID- 25724068
OWN - NLM
STAT- MEDLINE
DCOM- 20160208
LR  - 20181202
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 6
DP  - 2015 Feb 27
TI  - The cholesterol transporter ABCG1 links cholesterol homeostasis and tumour 
      immunity.
PG  - 6354
LID - 10.1038/ncomms7354 [doi]
AB  - ATP-binding cassette transporter G1 (ABCG1) promotes cholesterol efflux from 
      cells and regulates intracellular cholesterol homeostasis. Here we demonstrate a 
      role of ABCG1 as a mediator of tumour immunity. Abcg1(-/-) mice have dramatically 
      suppressed subcutaneous MB49-bladder carcinoma and B16-melanoma growth and 
      prolonged survival. We show that reduced tumour growth in Abcg1(-/-) mice is 
      myeloid cell intrinsic and is associated with a phenotypic shift of the 
      macrophages from a tumour-promoting M2 to a tumour-fighting M1 within the tumour. 
      Abcg1(-/-) macrophages exhibit an intrinsic bias towards M1 polarization with 
      increased NF-kappaB activation and direct cytotoxicity for tumour cells in vitro. 
      Overall, our study demonstrates that the absence of ABCG1 inhibits tumour growth 
      through modulation of macrophage function within the tumour, and illustrates a 
      link between cholesterol homeostasis and cancer.
FAU - Sag, Duygu
AU  - Sag D
AD  - Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, 
      La Jolla, California 92037, USA.
FAU - Cekic, Caglar
AU  - Cekic C
AD  - Department of Molecular Biology and Genetics, Bilkent University, Ankara 06800, 
      Turkey.
FAU - Wu, Runpei
AU  - Wu R
AD  - Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, 
      La Jolla, California 92037, USA.
FAU - Linden, Joel
AU  - Linden J
AD  - Division of Developmental Immunology, La Jolla Institute for Allergy and 
      Immunology, La Jolla, California 92037, USA.
FAU - Hedrick, Catherine C
AU  - Hedrick CC
AD  - Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, 
      La Jolla, California 92037, USA.
LA  - eng
GR  - P01 HL055798/HL/NHLBI NIH HHS/United States
GR  - R01 HL097368/HL/NHLBI NIH HHS/United States
GR  - S10 RR027366/RR/NCRR NIH HHS/United States
GR  - P01 HL55798/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150227
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (ABCG1 protein, mouse)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Lipoproteins)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/genetics/*metabolism
MH  - Animals
MH  - Carcinoma/*immunology/physiopathology
MH  - Cell Line, Tumor
MH  - Cholesterol/*metabolism
MH  - Flow Cytometry
MH  - Fluorescence
MH  - Homeostasis/*physiology
MH  - Lipoproteins/genetics/*metabolism
MH  - Macrophages/cytology/*immunology
MH  - Melanoma/*immunology/physiopathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Real-Time Polymerase Chain Reaction
MH  - Urinary Bladder Neoplasms/*immunology/physiopathology
PMC - PMC4347884
MID - NIHMS658095
EDAT- 2015/02/28 06:00
MHDA- 2016/02/09 06:00
PMCR- 2015/08/27
CRDT- 2015/02/28 06:00
PHST- 2014/04/21 00:00 [received]
PHST- 2015/01/22 00:00 [accepted]
PHST- 2015/02/28 06:00 [entrez]
PHST- 2015/02/28 06:00 [pubmed]
PHST- 2016/02/09 06:00 [medline]
PHST- 2015/08/27 00:00 [pmc-release]
AID - ncomms7354 [pii]
AID - 10.1038/ncomms7354 [doi]
PST - epublish
SO  - Nat Commun. 2015 Feb 27;6:6354. doi: 10.1038/ncomms7354.