PMID- 25724777
OWN - NLM
STAT- MEDLINE
DCOM- 20151111
LR  - 20150228
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 362
IP  - 4
DP  - 2015 Feb
TI  - Immunogenicity and protective effect of recombinant Brucella abortus Ndk (rNdk) 
      against a virulent strain B. abortus 544 infection in BALB/c mice.
LID - 10.1093/femsle/fnv003 [doi]
AB  - In this study, we particularly evaluated the protective effect of recombinant 
      protein encoded by Brucella abortus 544 ndk (nucleoside diphosphate kinase) gene 
      against B. abortus infection in the BALB/c mice. Cloning and expression of B. 
      abortus Ndk was accomplished by PCR amplification into a pMAL expression system, 
      and purification of a recombinant Ndk (rNdk). As for the determination of IgG 
      responses, rNdk induced vigorous IgG production, especially higher in IgG2a 
      compared to IgG1 with titers of 5.2 and 4.8, respectively, whereas titers of 
      these in mice immunized with MBP were 2.4 of IgG2a and 2.6 of IgG1. The analysis 
      of cytokine has revealed that rNdk can strongly induce production of IFN-gamma as 
      well as proinflammatory cytokines (TNF, MCP1 and IL-6) but not much IL-10, 
      suggesting rNdk elicited predominantly cell-mediated immune responses. 
      Furthermore, the spleen proliferation and bacterial burden in the spleen of rNdk 
      immunized mice were significantly lower than those of MBP-immunized mice against 
      virulent B. abortus challenge (P < 0.01). Conclusionly, rNdk immunization enables 
      to elicit both of the humoral and cellular response, ultimately enhancing 
      protection level in experimental mice, suggesting that rNdk of B. abortus might 
      be a useful candidate for subunit vaccine for brucellosis in animals.
CI  - (c) FEMS 2015. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Hop, Huynh Tan
AU  - Hop HT
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Gazwa, Jinju 660-701, Korea.
FAU - Simborio, Hannah Leah
AU  - Simborio HL
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Gazwa, Jinju 660-701, Korea.
FAU - Reyes, Alisha Wehdnesday Bernardo
AU  - Reyes AW
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Gazwa, Jinju 660-701, Korea.
FAU - Arayan, Lauren Togonon
AU  - Arayan LT
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Gazwa, Jinju 660-701, Korea.
FAU - Min, WonGi
AU  - Min W
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Gazwa, Jinju 660-701, Korea.
FAU - Lee, Hu Jang
AU  - Lee HJ
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Gazwa, Jinju 660-701, Korea.
FAU - Kim, Dong Hee
AU  - Kim DH
AD  - School of Medicine, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Chang, Hong Hee
AU  - Chang HH
AD  - Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 
      660-701, Republic of Korea.
FAU - Kim, Suk
AU  - Kim S
AD  - Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National 
      University, Gazwa, Jinju 660-701, Korea Institute of Agriculture and Life 
      Science, Gyeongsang National University, Jinju 660-701, Republic of Korea 
      kimsuk@gnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150113
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vaccines, Subunit)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.4.6 (Nucleoside-Diphosphate Kinase)
SB  - IM
MH  - Animals
MH  - Brucella abortus/*genetics/*immunology
MH  - Brucellosis/*immunology/prevention & control
MH  - Cloning, Molecular
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunity, Cellular
MH  - Immunity, Humoral
MH  - *Immunization
MH  - Immunoglobulin G/biosynthesis/immunology
MH  - Interferon-gamma/immunology/metabolism
MH  - Interleukin-10/immunology/metabolism
MH  - Interleukin-6/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nucleoside-Diphosphate Kinase/*genetics/*immunology
MH  - Recombinant Proteins/isolation & purification
MH  - Tumor Necrosis Factor-alpha/immunology/metabolism
MH  - Vaccines, Subunit
OTO - NOTNLM
OT  - Brucella abortus
OT  - nucleoside diphosphate kinase (Ndk)
OT  - protection
OT  - vaccine
EDAT- 2015/03/01 06:00
MHDA- 2015/11/12 06:00
CRDT- 2015/03/01 06:00
PHST- 2015/03/01 06:00 [entrez]
PHST- 2015/03/01 06:00 [pubmed]
PHST- 2015/11/12 06:00 [medline]
AID - fnv003 [pii]
AID - 10.1093/femsle/fnv003 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2015 Feb;362(4). doi: 10.1093/femsle/fnv003. Epub 2015 Jan 
      13.