PMID- 25725139
OWN - NLM
STAT- MEDLINE
DCOM- 20151130
LR  - 20210309
IS  - 1840-4812 (Electronic)
IS  - 1512-8601 (Print)
IS  - 1512-8601 (Linking)
VI  - 15
IP  - 1
DP  - 2015 Feb 5
TI  - Effect and mechanisms of zinc supplementation in protecting against diabetic 
      cardiomyopathy in a rat model of type 2 diabetes.
PG  - 14-20
LID - 10.17305/bjbms.2015.63 [doi]
AB  - Diabetic cardiomyopathy is a prominent cause of heart failure in patients with 
      diabetes mellitus. Currently, there is no specific treatment for diabetic 
      cardiomyopathy. This study aimed to investigate the effect and underlying 
      mechanisms of Zinc (Zn) supplementation in the protection against diabetic 
      cardiomyopathy in a rat model of type 2 diabetes mellitus (T2DM). T2DM-like 
      lesions in male Wistar rats were induced by introducing the high-fat diet and by 
      administration of streptozocin (STZ). After STZ induction, animals with fasting 
      plasma glucose level >/=16.7 mM were considered as diabetic, and randomly assigned 
      to the group receiving physiological saline (control) or ZnSO4 for 56 days. On 
      days 0, 7, 28 and 56 of treatment, animals were weighed, and their blood samples 
      were analyzed. On day 56, hemodynamic assessment was performed right before the 
      sacrifice of animals. Cardiac tissue specimens were collected and subjected to 
      pathologic assessment, metallothionein (MT) concentration measurement and Western 
      blot analysis of microtubule-associated protein light chain 3 (LC3), the marker 
      of autophagy, and glucose-regulated protein-78 (GRP78), an oxidative stress 
      marker. High-fat diet feeding followed by STZ administration resulted in weight 
      loss, hyperglycemia, polydipsia, polyphagia, hemodynamic anomalies and a 
      significant increase in the myocardial content of LC3 and GRP78 proteins, but not 
      in MT protein. Zn supplementation effectively attenuated all these aberrations 
      induced by high-fat diet and STZ. These findings suggest that Zn might be a 
      protective factor in diabetic cardiomyopathy, acting in two ways: at least 
      partially, through inhibiting autophagy and by endoplasmic reticulum stress.
FAU - Lu, Ying
AU  - Lu Y
AD  - Jilin University. luying1976star@hotmail.com.
FAU - Liu, Ya
AU  - Liu Y
FAU - Li, Hongyan
AU  - Li H
FAU - Wang, Xue
AU  - Wang X
FAU - Wu, Wenjie
AU  - Wu W
FAU - Gao, Lichao
AU  - Gao L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150205
PL  - Bosnia and Herzegovina
TA  - Bosn J Basic Med Sci
JT  - Bosnian journal of basic medical sciences
JID - 101200947
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (GRP78 protein, rat)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (LC3 protein, rat)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 5W494URQ81 (Streptozocin)
RN  - 9038-94-2 (Metallothionein)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Autophagy/drug effects
MH  - Biomarkers/metabolism
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Type 2/*complications/*etiology
MH  - Diabetic Cardiomyopathies/metabolism/*prevention & control
MH  - Diet, High-Fat/adverse effects
MH  - *Dietary Supplements
MH  - Disease Models, Animal
MH  - Endoplasmic Reticulum Stress/drug effects
MH  - Heat-Shock Proteins/metabolism
MH  - Male
MH  - Metallothionein/metabolism
MH  - Microtubule-Associated Proteins/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Streptozocin/*adverse effects
MH  - Treatment Outcome
MH  - Zinc/*administration & dosage/pharmacology/*therapeutic use
PMC - PMC4365671
EDAT- 2015/03/01 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/02/01
CRDT- 2015/03/01 06:00
PHST- 2014/08/04 00:00 [received]
PHST- 2014/12/12 00:00 [accepted]
PHST- 2014/09/23 00:00 [revised]
PHST- 2015/03/01 06:00 [entrez]
PHST- 2015/03/01 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/02/01 00:00 [pmc-release]
AID - BJBMS-1-14 [pii]
AID - 10.17305/bjbms.2015.63 [doi]
PST - epublish
SO  - Bosn J Basic Med Sci. 2015 Feb 5;15(1):14-20. doi: 10.17305/bjbms.2015.63.