PMID- 25729546
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150302
LR  - 20201001
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 17
IP  - 10
DP  - 2014 Oct
TI  - Engraftment of plasma membrane vesicles into liposomes: A new method for 
      designing of liposome-based vaccines.
PG  - 772-8
AB  - OBJECTIVES: One of the major challenges in the field of vaccine design is 
      choosing immunogenic antigens which can induce a proper immune response against 
      complex targets like malignant cells or recondite diseases caused by protozoan 
      parasites such as leishmaniasis. The aim of this study was to find a way to 
      construct artificial liposome-based cells containing fragments of target's cell 
      membrane. This structure not only mimics the real biological properties of 
      proteins in the cell membrane of target cells, but also may induce the required 
      immune responses, which culminate in eradication of target cells. MATERIALS AND 
      METHODS: Five different techniques have been investigated to engraft the plasma 
      membrane's vesicles (PMVs) derived from a characterized Leishmania parasite into 
      liposomes. The most efficient method was tested again on the PMVs derived from 
      well-known breast cancer cell line SK-BR-3. The percentage of engraftment was 
      determined by two-color flowcytometry after staining the engrafted 
      dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine DiI-labeled liposomes with 
      FITC-labeled PMVs. RESULTS: Among the investigated techniques, freeze-drying 
      method with 91+/-2% and 90+/-3% of engraftment for Leishmania and SK-BR-3 derived 
      PMVs, respectively, showed superiority over the other methods. In addition, after 
      9 weeks storage in refrigerator, freeze-dried fused particles kept their original 
      size (660+/-350 nm) and fusion efficiency (94+/-3%). CONCLUSION: Among five different 
      engraftment techniques, freeze-drying is preferred over the other methods due to 
      its simplicity, more fusion efficiency and stability of produced particles during 
      storage.
FAU - Samiei, Afshin
AU  - Samiei A
AD  - Department of Immunology, Autoimmune Diseases Research Center, Shiraz University 
      of Medical Sciences, Shiraz, Iran.
FAU - Tamadon, Ali Mohammad
AU  - Tamadon AM
AD  - Department of Pharmaceutical Biotechnology Faculty of Pharmacy, Shiraz University 
      of Medical Sciences, Shiraz, Iran.
FAU - Samani, Soliman Mohammadi
AU  - Samani SM
AD  - Department of Pharmaceutical Biotechnology Faculty of Pharmacy, Shiraz University 
      of Medical Sciences, Shiraz, Iran.
FAU - Manolios, Nicholas
AU  - Manolios N
AD  - School of Medicine, University of Sydney, Sydney, Australia.
FAU - Sarvestani, Eskandar Kamali
AU  - Sarvestani EK
AD  - Department of Immunology, Autoimmune Diseases Research Center, Shiraz University 
      of Medical Sciences, Shiraz, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC4340985
OTO - NOTNLM
OT  - DiI
OT  - Fused particles
OT  - Leishmania
OT  - Liposome
OT  - Plasma membrane vesicle
OT  - SK-BR-3
EDAT- 2015/03/03 06:00
MHDA- 2015/03/03 06:01
PMCR- 2014/10/01
CRDT- 2015/03/03 06:00
PHST- 2013/12/17 00:00 [received]
PHST- 2014/03/08 00:00 [accepted]
PHST- 2015/03/03 06:00 [entrez]
PHST- 2015/03/03 06:00 [pubmed]
PHST- 2015/03/03 06:01 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - IJBMS-17-772 [pii]
PST - ppublish
SO  - Iran J Basic Med Sci. 2014 Oct;17(10):772-8.