PMID- 2572965
OWN - NLM
STAT- MEDLINE
DCOM- 19891208
LR  - 20190820
IS  - 0166-6851 (Print)
IS  - 0166-6851 (Linking)
VI  - 36
IP  - 1
DP  - 1989 Aug
TI  - Modulation of Trypanosoma cruzi adhesion to host muscle cell membranes by ligands 
      of muscarinic cholinergic and beta adrenergic receptors.
PG  - 41-50
AB  - Plasma membrane vesicles (PMVs) of Trypanosoma cruzi adhered to L6 myoblast host 
      cells as a function of time and concentration in saturation phenomena in a 
      similar fashion to that reported in a previous publication. The initial adhesion 
      rate (A0) of T. cruzi PMVs to L6 myoblasts in tissue culture was inhibited by 
      acetylcholine (10(-5) M), isoproterenol (10(-5) M) and norepinephrine (10(-8) M) 
      (range 29.1-50.3% of control). Atropine, the antagonist of muscarinic cholinergic 
      receptors (10(-5) M), and propranolol or pindolol, the antagonists of 
      beta-adrenergic receptors (10(-5) M), were also equal inhibitors of the T. cruzi 
      PMV to L6 myoblast adhesion rate (range 26.1 to 55.5% of control). The 
      alpha-adrenergic receptor ligands yohimbine and phentolamine (10(-5) M) showed no 
      A0 inhibitory activity in similar assays. The interaction of T. cruzi PMVs with 
      type I host muscle sarcolemma receptors was clearly defined in assays which used 
      porcine heart atrial membranes (PAMs) immobilized on cationic polyacrylamide 
      beads. In this parasite membrane-host cell membrane assay system, 10(-10) M 
      atropine and 5 x 10(-9) M propranolol produced a shift of an S-shaped T. cruzi 
      PMV to PAM saturation isotherm to the right, suggesting that a negative 
      cooperative interaction was produced between the intramembrane ligand binding 
      site and another, surface heterotropic T. cruzi PMV adhesion site. Atropine and 
      propranolol were equieffective inhibitors of the T. cruzi striated muscle 
      sarcolemma recognition process, raising the possibility that T. cruzi attachment 
      molecules annexed pairs of muscarinic cholinergic and beta-adrenergic receptors 
      to effect adhesion of the two membrane surfaces.
FAU - von Kreuter, B F
AU  - von Kreuter BF
AD  - Department of Pathology, Cornell University Medical College, New York, NY 10021.
FAU - Santos-Buch, C A
AU  - Santos-Buch CA
LA  - eng
GR  - 1-R01-34221/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Adrenergic beta-Antagonists)
RN  - 0 (Receptors, Adrenergic)
RN  - 0 (Receptors, Muscarinic)
SB  - IM
MH  - Adrenergic beta-Agonists/*pharmacology
MH  - Adrenergic beta-Antagonists/*pharmacology
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Line
MH  - Cell Membrane/drug effects/metabolism/parasitology
MH  - Models, Chemical
MH  - Receptors, Adrenergic/drug effects/*physiology
MH  - Receptors, Muscarinic/drug effects/*physiology
MH  - Sarcolemma/drug effects/*metabolism/parasitology
MH  - Swine
MH  - Trypanosoma cruzi/drug effects/*metabolism
EDAT- 1989/08/01 00:00
MHDA- 1989/08/01 00:01
CRDT- 1989/08/01 00:00
PHST- 1989/08/01 00:00 [pubmed]
PHST- 1989/08/01 00:01 [medline]
PHST- 1989/08/01 00:00 [entrez]
AID - 0166-6851(89)90198-9 [pii]
AID - 10.1016/0166-6851(89)90198-9 [doi]
PST - ppublish
SO  - Mol Biochem Parasitol. 1989 Aug;36(1):41-50. doi: 10.1016/0166-6851(89)90198-9.