PMID- 25730044
OWN - NLM
STAT- MEDLINE
DCOM- 20160125
LR  - 20150303
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 1
DP  - 2015 Feb 6
TI  - Quantitative real-time polymerase chain reaction as an efficient molecular tool 
      for detecting minimal residual disease in Moroccan chronic myeloid leukemia 
      patients.
PG  - 1044-55
LID - 10.4238/2015.February.6.8 [doi]
AB  - Chronic myeloid leukemia (CML) is characterized by BCR-ABL translocation and an 
      increased number and migration of immature myeloid cells into the peripheral 
      blood. The detection limit of the BCR-ABL transcript, particularly after 
      treatment, is controversial. In the present study, we used quantitative real-time 
      reverse transcription-polymerase chain reaction (RT-qPCR) to monitor BCR-ABL 
      expression in Moroccan CML patients undergoing imatinib treatment, and compared 
      the results with those of conventional PCR and fluorescence in situ hybridization 
      (FISH). The aim of this study was to establish a new molecular tool for in vitro 
      diagnosis of CML. In a retrospective comparative analysis, 20 CML Moroccan 
      patients who had received imatinib treatment (N = 20) were analyzed by real-time 
      PCR, conventional RT-PCR, and FISH. Half of the samples analyzed (N = 10) were 
      positive for BCR-ABL gene expression, while the other half (N = 10) were negative 
      according to conventional PCR. Interestingly, 5 of the 10 samples shown to be 
      negative by conventional PCR showed positive expression of the BCR-ABL gene 
      according to RT-qPCR. The RT-qPCR results were confirmed by FISH, which revealed 
      a high concordance (100%) rate. We found that real-time RT-qPCR is more reliable 
      and should be used in Moroccan biomedical analysis laboratories to monitor CML 
      progression, particularly for minimal residual disease, following imatinib 
      treatment.
FAU - Moumen, A
AU  - Moumen A
AD  - Moroccan Foundation for Advanced Science, Innovation and Research Rabat, Morocco.
FAU - Dehbi, H
AU  - Dehbi H
AD  - Laboratory of Human Genetics, Faculty of Medicine, Casablanca, Morocco.
FAU - Kottwitz, D
AU  - Kottwitz D
AD  - Moroccan Foundation for Advanced Science, Innovation and Research Rabat, Morocco.
FAU - El Amrani, M
AU  - El Amrani M
AD  - Moroccan Foundation for Advanced Science, Innovation and Research Rabat, Morocco.
FAU - Bouchoutrouch, N
AU  - Bouchoutrouch N
AD  - Moroccan Foundation for Advanced Science, Innovation and Research Rabat, Morocco.
FAU - El Hadi, H
AU  - El Hadi H
AD  - Moroccan Foundation for Advanced Science, Innovation and Research Rabat, Morocco.
FAU - Quessar, A
AU  - Quessar A
AD  - Hematology Department, Ibn Rochd University Hospital, Casablanca, Morocco.
FAU - Benchekroun, S
AU  - Benchekroun S
AD  - Hematology Department, Ibn Rochd University Hospital, Casablanca, Morocco.
FAU - Nadifi, S
AU  - Nadifi S
AD  - Laboratory of Human Genetics, Faculty of Medicine, Casablanca, Morocco.
FAU - Sefrioui, H
AU  - Sefrioui H
AD  - Moroccan Foundation for Advanced Science, Innovation and Research Rabat, Morocco 
      h.sefrioui@mascir.com.
LA  - eng
PT  - Journal Article
DEP - 20150206
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 8A1O1M485B (Imatinib Mesylate)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Fusion Proteins, bcr-abl/blood/*genetics
MH  - Humans
MH  - Imatinib Mesylate/adverse effects
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*blood/drug 
      therapy/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Morocco
MH  - Neoplasm, Residual/*blood/chemically induced/*genetics/pathology
MH  - Pathology, Molecular
MH  - Real-Time Polymerase Chain Reaction
MH  - Retrospective Studies
EDAT- 2015/03/03 06:00
MHDA- 2016/01/26 06:00
CRDT- 2015/03/03 06:00
PHST- 2015/03/03 06:00 [entrez]
PHST- 2015/03/03 06:00 [pubmed]
PHST- 2016/01/26 06:00 [medline]
AID - gmr4436 [pii]
AID - 10.4238/2015.February.6.8 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Feb 6;14(1):1044-55. doi: 10.4238/2015.February.6.8.