PMID- 25736782
OWN - NLM
STAT- MEDLINE
DCOM- 20160418
LR  - 20150703
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 15
IP  - 13
DP  - 2015 Jul
TI  - Proteomic and network analysis of proteins regulated by REX1 in human embryonic 
      stem cells.
PG  - 2220-9
LID - 10.1002/pmic.201400510 [doi]
AB  - Recent studies have suggested that REX1 (reduced expression 1) plays an important 
      role in pluripotency, proliferation, and differentiation. However, the molecular 
      mechanisms involved in REX1-dependent regulation of diverse cellular processes 
      remain unclear. To elucidate the regulatory functions of REX1 in human embryonic 
      stem cells (hESCs), comparative proteomic analysis was performed on REX1 RNAi 
      specifically silenced hESCs. Analysis of the proteome via nano-LC-MS/MS 
      identified 140 differentially expressed proteins (DEPs) displaying a >2-fold 
      difference in expression level between control and REX1 knockdown (KD) hESCs, 
      which were then compared with transcriptome data and validated by quantitative 
      real-time RT-PCR and Western blotting. These DEPs were analyzed by GO, pathway, 
      and functional clustering analyses to determine the molecular functions of the 
      proteins and pathways regulated by REX1. The REX1 KD-mediated DEPs mapped to 
      major biological processes involved in the regulation of ribosome-mediated 
      translation and mitochondrial function. Functional network analysis revealed a 
      highly interconnected network among these DEPs and indicated that these 
      interconnected proteins are predominantly involved in translation and the 
      regulation of mitochondrial organization. These findings regarding REX1-mediated 
      regulatory network have revealed the contributions of REX1 to maintaining the 
      status of hESCs and have improved our understanding of the molecular events that 
      underlie the fundamental properties of hESCs.
CI  - (c) 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Son, Mi-Young
AU  - Son MY
AD  - Stem Cell Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Gwahangno, Yuseong-gu, Daejeon, Republic of Korea.
AD  - Department of functional genomics, University of Science & Technology, Gajungro, 
      Yuseong-gu, Daejeon, Republic of Korea.
FAU - Kwak, Jae Eun
AU  - Kwak JE
AD  - Stem Cell Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Gwahangno, Yuseong-gu, Daejeon, Republic of Korea.
FAU - Kim, Young-Dae
AU  - Kim YD
AD  - Stem Cell Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Gwahangno, Yuseong-gu, Daejeon, Republic of Korea.
FAU - Cho, Yee Sook
AU  - Cho YS
AD  - Stem Cell Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), Gwahangno, Yuseong-gu, Daejeon, Republic of Korea.
AD  - Department of functional genomics, University of Science & Technology, Gajungro, 
      Yuseong-gu, Daejeon, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150423
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (ZFP42 protein, human)
SB  - IM
CIN - Proteomics. 2015 Jul;15(13):2147-9. PMID: 26058466
MH  - Blotting, Western
MH  - Human Embryonic Stem Cells/*metabolism
MH  - Humans
MH  - Kruppel-Like Transcription Factors/genetics/*metabolism
MH  - *Proteomics
MH  - RNA Interference
OTO - NOTNLM
OT  - Cell biology
OT  - Human embryonic stem cells
OT  - Pluripotency
OT  - Quantitative proteomics
OT  - REX1
EDAT- 2015/03/05 06:00
MHDA- 2016/04/19 06:00
CRDT- 2015/03/05 06:00
PHST- 2014/10/31 00:00 [received]
PHST- 2015/01/19 00:00 [revised]
PHST- 2015/02/26 00:00 [accepted]
PHST- 2015/03/05 06:00 [entrez]
PHST- 2015/03/05 06:00 [pubmed]
PHST- 2016/04/19 06:00 [medline]
AID - 10.1002/pmic.201400510 [doi]
PST - ppublish
SO  - Proteomics. 2015 Jul;15(13):2220-9. doi: 10.1002/pmic.201400510. Epub 2015 Apr 
      23.