PMID- 25738414
OWN - NLM
STAT- MEDLINE
DCOM- 20160105
LR  - 20181202
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 12
IP  - 1
DP  - 2015 Jul
TI  - Co-culture with bone marrow stromal cells protects PC12 neuronal cells from tumor 
      necrosis factor-alpha-induced apoptosis by inhibiting the tumor necrosis factor 
      receptor/caspase signaling pathway.
PG  - 261-6
LID - 10.3892/mmr.2015.3421 [doi]
AB  - Bone marrow stromal cells (BMSCs), derived from the mesoderm, have been applied 
      in the repair and reconstruction of injured tissues. The present study was 
      conducted to explore the effects of BMSCs on cell viability of tumor necrosis 
      factor-alpha (TNF-alpha)-stimulated PC12 cells. PC12 cells were co-cultured with BMSCs 
      under TNF-alpha treatment, with normal PC12 cells as controls. Results from an MTT 
      assay indicated that BMSCs significantly increased cell growth and proliferation 
      of TNF-alpha-treated PC12 cells (survival rates were 56.71 and 76.86% for the 
      positive control (PC) and co-culture group, respectively). Furthermore, Annexin 
      V/propidium iodide staining and flow cytometric analysis demonstrated that TNF-alpha 
      increased PC12-cell apoptosis from 3.49 to 40.74% in the negative control and PC 
      group, and the apoptotic rate was significantly reduced upon co-culture with 
      BMSCs to 16.97%. In addition, data from reverse transcription-quantitative 
      polymerase chain reaction and western blot analyses illustrated that 
      TNF-alpha-induced upregulation in TNF receptor (TNFR)-1 (TNFR1) and caspase-8 
      expression in PC12 cells were partially reversed by co-culture with BMSCs. In 
      conclusion, the present study suggested that BMSCs protect PC12 cells against 
      stimulation with TNF-alpha, which is partially mediated through the TNFR/caspase 
      signaling pathway. The results of the present study also suggested a therapeutic 
      use of BMSCs in clinical neurodegenerative diseases.
FAU - Li, Li
AU  - Li L
AD  - Department of Neurology, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Neurology, The 463th Hospital of the Chinese People's Liberation 
      Army, Shenyang, Liaoning 110042, P.R. China.
FAU - Tang, Ling
AU  - Tang L
AD  - Department of Neurology, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Yu, Xin
AU  - Yu X
AD  - Department of Clinical Pharmacy, China Medical University, Shenyang, Liaoning 
      110001, P.R. China.
FAU - Sui, Yi
AU  - Sui Y
AD  - Department of Neurology, Shenyang First People's Hospital, Shenyang, Liaoning 
      110041, P.R. China.
FAU - Zhang, Chaodong
AU  - Zhang C
AD  - Department of Neurology, The First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20150304
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Annexin A5)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Tnfrsf1a protein, rat)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 36015-30-2 (Propidium)
RN  - EC 3.4.22.- (Casp8 protein, rat)
RN  - EC 3.4.22.- (Caspase 8)
SB  - IM
MH  - Animals
MH  - Annexin A5
MH  - Apoptosis/drug effects
MH  - Bone Marrow Cells/cytology/*drug effects/immunology
MH  - Caspase 8/*genetics/metabolism
MH  - Cell Communication
MH  - Cell Differentiation
MH  - Coculture Techniques
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*drug effects/immunology
MH  - Neurons/cytology/*drug effects/immunology
MH  - PC12 Cells
MH  - Propidium
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Tumor Necrosis Factor, Type I/antagonists & 
      inhibitors/*genetics/metabolism
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2015/03/05 06:00
MHDA- 2016/01/06 06:00
CRDT- 2015/03/05 06:00
PHST- 2014/04/17 00:00 [received]
PHST- 2015/01/02 00:00 [accepted]
PHST- 2015/03/05 06:00 [entrez]
PHST- 2015/03/05 06:00 [pubmed]
PHST- 2016/01/06 06:00 [medline]
AID - 10.3892/mmr.2015.3421 [doi]
PST - ppublish
SO  - Mol Med Rep. 2015 Jul;12(1):261-6. doi: 10.3892/mmr.2015.3421. Epub 2015 Mar 4.