PMID- 25738636
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20201209
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 33
IP  - 5
DP  - 2015 May
TI  - Effects of the knockdown of death-associated protein 3 expression on cell 
      adhesion, growth and migration in breast cancer cells.
PG  - 2575-82
LID - 10.3892/or.2015.3825 [doi]
AB  - The death-associated protein 3 (DAP3) is a highly conserved phosphoprotein 
      involved in the regulation of autophagy. A previous clinical study by our group 
      suggested an association between low DAP3 expression and clinicopathological 
      parameters of human breast cancer. In the present study, we intended to determine 
      the role of DAP3 in cancer cell behaviour in the context of human breast cancer. 
      We developed knockdown sub-lines of MCF7 and MDA-MB-231, and performed growth, 
      adhesion, invasion assays and electric cell-substrate impedance sensing (ECIS) 
      studies of post-wound migration of the cells. In addition, we studied the mRNA 
      expression of caspase 8 and 9, death ligand signal enhancer (DELE), IFN-beta 
      promoter stimulator 1 (IPS1), cyclin D1 and p21 in the control and knockdown 
      sub-lines. The knockdown sub-lines of MCF7 and MDA-MB-231 had significantly 
      increased adhesion and decreased growth when compared to the controls. 
      Furthermore, invasion and migration were significantly increased in the 
      MDA-MB-231DAP3kd cells vs. the controls. The expression of caspase 9 and IPS1, 
      known components of the apoptosis pathway, were significantly reduced in the 
      MCF7DAP3kd cells (p=0.05 and p=0.003, respectively). We conclude that DAP3 
      silencing contributes to breast carcinogenesis by increasing cell adhesion, 
      migration and invasion. It is possible that this may be due to the activity of 
      focal adhesion kinase further downstream of the anoikis pathway. Further research 
      in this direction would be beneficial in increasing our understanding of the 
      mechanisms underlying human breast cancer.
FAU - Wazir, Umar
AU  - Wazir U
AD  - The London Breast Institute, Princess Grace Hospital, London, UK.
FAU - Sanders, Andrew J
AU  - Sanders AJ
AD  - Cardiff University-Peking University Cancer Institute (CUPUCI), Cardiff 
      University School of Medicine, Cardiff University, Cardiff, Wales, UK.
FAU - Wazir, Ahmad M A
AU  - Wazir AM
AD  - Peshawar Medical College, Peshawar, Pakistan.
FAU - Ye, Lin
AU  - Ye L
AD  - Cardiff University-Peking University Cancer Institute (CUPUCI), Cardiff 
      University School of Medicine, Cardiff University, Cardiff, Wales, UK.
FAU - Jiang, Wen G
AU  - Jiang WG
AD  - Cardiff University-Peking University Cancer Institute (CUPUCI), Cardiff 
      University School of Medicine, Cardiff University, Cardiff, Wales, UK.
FAU - Ster, Irina C
AU  - Ster IC
AD  - Department of Breast Surgery, St. George's Hospital and Medical School, 
      University of London, London, UK.
FAU - Sharma, Anup K
AU  - Sharma AK
AD  - Department of Breast Surgery, St. George's Hospital and Medical School, 
      University of London, London, UK.
FAU - Mokbel, Kefah
AU  - Mokbel K
AD  - The London Breast Institute, Princess Grace Hospital, London, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150302
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (DAP3 protein, human)
RN  - 0 (DELE1 protein, human)
RN  - 0 (MAVS protein, human)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribosomal Proteins)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EC 3.4.22.- (Caspase 9)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics
MH  - Apoptosis/genetics
MH  - Apoptosis Regulatory Proteins/*genetics
MH  - Breast Neoplasms/*genetics/pathology
MH  - Caspase 8/genetics
MH  - Caspase 9/genetics
MH  - Cell Adhesion/*genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/*genetics
MH  - Cell Proliferation/*genetics
MH  - Cyclin D1/genetics
MH  - Cyclin-Dependent Kinase Inhibitor p21/genetics
MH  - Female
MH  - Focal Adhesion Protein-Tyrosine Kinases/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - MCF-7 Cells
MH  - Mitochondrial Proteins/genetics
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - RNA-Binding Proteins
MH  - Ribosomal Proteins/*genetics
MH  - Signal Transduction/genetics
EDAT- 2015/03/05 06:00
MHDA- 2015/12/17 06:00
CRDT- 2015/03/05 06:00
PHST- 2014/12/30 00:00 [received]
PHST- 2015/02/04 00:00 [accepted]
PHST- 2015/03/05 06:00 [entrez]
PHST- 2015/03/05 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
AID - 10.3892/or.2015.3825 [doi]
PST - ppublish
SO  - Oncol Rep. 2015 May;33(5):2575-82. doi: 10.3892/or.2015.3825. Epub 2015 Mar 2.