PMID- 25739187
OWN - NLM
STAT- MEDLINE
DCOM- 20150910
LR  - 20150305
IS  - 0869-2092 (Print)
IS  - 0869-2092 (Linking)
VI  - 77
IP  - 12
DP  - 2014
TI  - [Determining the relative content of cisplatin in cells by acridine orange 
      fluorescence intensity].
PG  - 14-6
AB  - The fluorescence intensity of acridine orange (AO) dye bound to living human 
      leukocytes, rat leukocytes, and Pliss lymphosarcoma cells of rat has been studied 
      by fluorescence microscopy. The cell suspension of each test was divided into two 
      parts, to one of which the drug cisplatin was added to a final concentration of 
      0.1 mug/mL and the mixture was incubated for 20 - 60 min. Acridine orange was then 
      added and the average intensity of red and green fluorescence of cells was 
      determined. The brightness of AO in the green region of the spectrum was reduced 
      approximately 3-fold (p = 0.001) in cells with cisplatin. In the red region of 
      the fluorescence, differences in the brightness of cells with and without 
      cisplatin were not observed. These data indicate that a decrease in the 
      brightness of green fluorescence of AO can be a test for the presence of 
      cisplatin in cells.
FAU - Goroshinskaia, I A
AU  - Goroshinskaia IA
FAU - Tarnopol'skaia, O V
AU  - Tarnopol'skaia OV
FAU - Kachesova, P S
AU  - Kachesova PS
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Eksp Klin Farmakol
JT  - Eksperimental'naia i klinicheskaia farmakologiia
JID - 9215981
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Fluorescent Dyes)
RN  - F30N4O6XVV (Acridine Orange)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - Animals
MH  - Antineoplastic Agents/metabolism/*pharmacokinetics
MH  - Cell Culture Techniques
MH  - Cell Line, Tumor
MH  - Cisplatin/metabolism/*pharmacokinetics
MH  - Fluorescent Dyes/*chemistry
MH  - Humans
MH  - Leukocytes/*metabolism
MH  - Microscopy, Fluorescence
MH  - Rats
EDAT- 2014/01/01 00:00
MHDA- 2015/09/12 06:00
CRDT- 2015/03/06 06:00
PHST- 2015/03/06 06:00 [entrez]
PHST- 2014/01/01 00:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
PST - ppublish
SO  - Eksp Klin Farmakol. 2014;77(12):14-6.