PMID- 25739351
OWN - NLM
STAT- MEDLINE
DCOM- 20160211
LR  - 20220321
IS  - 1573-5168 (Electronic)
IS  - 0920-1742 (Linking)
VI  - 41
IP  - 3
DP  - 2015 Jun
TI  - Spatial learning associated with stimulus response in goldfish Carassius auratus: 
      relationship to activation of CREB signalling.
PG  - 685-94
LID - 10.1007/s10695-015-0038-9 [doi]
AB  - Earlier, we reported spatial learning ability in goldfish (Carassius auratus) by 
      using spatial paradigm with food reward. Therefore, we hypothesized that goldfish 
      may use associated cue to integrate "where" and "what" for spatial memory. To 
      test our hypothesis, we first trained goldfish to learn to cross the gate1, which 
      is associated with spatial task. Subsequently, they were trained to learn to 
      enter the task chamber and to identify the food reward chamber associated with 
      visual cue (red/green light). Red and green lights were positioned randomly for 
      each trial but always the food reward was kept in green chamber. In addition, to 
      elucidate the role of the signalling cascade in spatial memory associated with 
      visual cue, nicotinamide (NAM, 1000 mg/kg, i.p), a NAD(+) precursor, was used to 
      inhibit the Sirtuin 1 (SIRT1) cyclic AMP response element binding protein (CREB) 
      pathway. Fishes were trained for 5 days in a maze after treating with either 
      vehicle (VEH, DD H2O) or NAM, and then, they were individually tested for memory. 
      We found that VEH-treated fish learned and recalled the task successfully by 
      showing less latency and making more correct choices than NAM-treated group. 
      Subsequent analysis showed that NAM treatment significantly down-regulated the 
      phosphorylation of extracellular signal-regulated kinase (ERK1/2), CREB, 
      expression of SirT1 and brain-derived neurotrophic factor (Bdnf) in 
      telencephalon. Taken together, our results provide behavioural evidence of 
      spatial memory associated with visual cue in C. auratus, which could be regulated 
      by ERK1/2-CREB-SirT1-Bdnf pathway.
FAU - Rajan, Koilmani Emmanuvel
AU  - Rajan KE
AD  - Department of Animal Science, School of Life Sciences, Bharathidasan University, 
      Tiruchirappalli, 620 024, India, emmanuvel1972@yahoo.com.
FAU - Thangaleela, Subramanian
AU  - Thangaleela S
FAU - Balasundaram, Chellam
AU  - Balasundaram C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150305
PL  - Netherlands
TA  - Fish Physiol Biochem
JT  - Fish physiology and biochemistry
JID - 100955049
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 25X51I8RD4 (Niacinamide)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Conditioning, Psychological
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Goldfish/*physiology
MH  - MAP Kinase Signaling System/*physiology
MH  - Maze Learning/*physiology
MH  - Niacinamide/pharmacology
MH  - Phosphorylation/drug effects
MH  - Photic Stimulation
MH  - Reward
MH  - Spatial Memory/*physiology
MH  - Telencephalon/drug effects
EDAT- 2015/03/06 06:00
MHDA- 2016/02/13 06:00
CRDT- 2015/03/06 06:00
PHST- 2014/05/01 00:00 [received]
PHST- 2015/02/23 00:00 [accepted]
PHST- 2015/03/06 06:00 [entrez]
PHST- 2015/03/06 06:00 [pubmed]
PHST- 2016/02/13 06:00 [medline]
AID - 10.1007/s10695-015-0038-9 [doi]
PST - ppublish
SO  - Fish Physiol Biochem. 2015 Jun;41(3):685-94. doi: 10.1007/s10695-015-0038-9. Epub 
      2015 Mar 5.