PMID- 25739957
OWN - NLM
STAT- MEDLINE
DCOM- 20160412
LR  - 20181113
IS  - 1348-4400 (Electronic)
IS  - 0916-8818 (Print)
IS  - 0916-8818 (Linking)
VI  - 61
IP  - 3
DP  - 2015
TI  - Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen 
      quality.
PG  - 185-90
LID - 10.1262/jrd.2014-140 [doi]
AB  - Sperm DNA damage affects the conception rate resulting from human assisted 
      reproduction technology. The objective of this study was to adapt the terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to 
      provide a quality parameter for bull semen based on the detection of sperm DNA 
      damage. Fresh semen was collected from two Japanese Black bulls (A, B) several 
      times over the course of a year, and the percentage of TUNEL-positive spermatozoa 
      (sperm TUNEL index) was determined. Individual differences in semen were detected 
      using the sperm TUNEL index in these bulls (P < 0.01). The sperm TUNEL index of 
      cryopreserved semen obtained from test-mated Japanese Black (n = 30, including 
      two bulls with a conception rate lower than 10%) and Holstein (n = 34) bulls were 
      analyzed. The average sperm TUNEL index and conception rate resulting from 
      artificial insemination (AI) were 4.7% and 55.7% for Japanese Black, and 4.9% and 
      39.5% for Holstein, respectively. A weak negative correlation between sperm TUNEL 
      index and conception rate was observed in Holstein bulls (P < 0.05). Semen 
      samples from six bulls with more than 10% sperm TUNEL index were studied, and 
      these samples showed low sperm viability. However, semen resulting in a very low 
      conception rate did not have a high sperm TUNEL index. Although it would be 
      difficult to predict a low conception rate resulting from AI using the sperm 
      TUNEL index alone, the index can be used as an additional parameter to provide a 
      more comprehensive description of semen quality.
FAU - Takeda, Kumiko
AU  - Takeda K
AD  - Animal Breeding and Reproduction Research Division, NARO Institute of Livestock 
      and Grassland Science, Ibaraki 305-0901, Japan.
FAU - Uchiyama, Kyoko
AU  - Uchiyama K
FAU - Kinukawa, Masashi
AU  - Kinukawa M
FAU - Tagami, Takahiro
AU  - Tagami T
FAU - Kaneda, Masahiro
AU  - Kaneda M
FAU - Watanabe, Shinya
AU  - Watanabe S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150127
PL  - Japan
TA  - J Reprod Dev
JT  - The Journal of reproduction and development
JID - 9438792
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cryopreservation
MH  - *DNA Damage
MH  - Fertilization
MH  - In Situ Nick-End Labeling/*methods
MH  - Male
MH  - *Semen
MH  - *Semen Analysis
MH  - Semen Preservation/methods
MH  - Species Specificity
MH  - Sperm Motility
MH  - Spermatozoa/*pathology
PMC - PMC4498374
EDAT- 2015/03/06 06:00
MHDA- 2016/04/14 06:00
PMCR- 2015/06/01
CRDT- 2015/03/06 06:00
PHST- 2015/03/06 06:00 [entrez]
PHST- 2015/03/06 06:00 [pubmed]
PHST- 2016/04/14 06:00 [medline]
PHST- 2015/06/01 00:00 [pmc-release]
AID - 2014-140 [pii]
AID - 10.1262/jrd.2014-140 [doi]
PST - ppublish
SO  - J Reprod Dev. 2015;61(3):185-90. doi: 10.1262/jrd.2014-140. Epub 2015 Jan 27.