PMID- 25740137
OWN - NLM
STAT- MEDLINE
DCOM- 20151229
LR  - 20190918
IS  - 0973-7138 (Electronic)
IS  - 0250-5991 (Linking)
VI  - 40
IP  - 1
DP  - 2015 Mar
TI  - Lack of IL-6 increases blood-brain barrier permeability in fungal meningitis.
PG  - 7-12
AB  - The pathogenesis of increased blood-brain barrier permeability during 
      Cryptococcus meningitis is still largely unknown. Interleukin (IL-6) is a 
      multifunctional cytokine, and numerous studies have shown that IL-6 influences 
      the integrity of the blood-brain barrier. In this study we investigated the role 
      of IL-6 in Cryptococcus meningitis. First, wild-type or IL-6(-/-) mice were 
      injected with Cryptococcus neoformans (C. neoformans) and the survival time in 
      both groups was recorded. Second, the number of fungi was measured in the brains 
      of IL-6(-/-) wild-type mice. Finally, the blood-brain barrier permeability index 
      was detected in infected IL-6(-/-) mice treated with recombinant human IL-6. The 
      blood-brain barrier permeability index was measured in infected wild-type mice 
      treated with anti-IL-6 antibodies as well. The survival of IL-6(-/-) mice 
      injected with C. neoformans was significantly lower than that of identically 
      challenged wild-type mice. The infected IL-6(-/-) mice had significantly larger 
      brain fungal burdens than wild-type mice. Furthermore, increased blood-brain 
      barrier index was found in infected IL-6(-/-) mice when compared with that in 
      infected control mice. Similar results were obtained when mice challenged with C. 
      neoformans were treated systemically with neutralizing anti-IL-6 antibodies, 
      resulting in an elevation of vascular permeability. Our data revealed that IL-6 
      reduced the blood-brain barrier permeability during Cryptococcus meningitis, and 
      it might provide an explanation for the significantly lower survival of infected 
      IL-6(-/-) mice.
FAU - Li, Xiang
AU  - Li X
AD  - Department of Pharmacy, First Affiliated Hospital of Chinese PLA General 
      Hospital, Beijing 100048, China, lxbj@hotmail.com.
FAU - Liu, Guiyang
AU  - Liu G
FAU - Ma, Jianli
AU  - Ma J
FAU - Zhou, Liang
AU  - Zhou L
FAU - Zhang, Qingzhe
AU  - Zhang Q
FAU - Gao, Lei
AU  - Gao L
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Biosci
JT  - Journal of biosciences
JID - 8100809
RN  - 0 (Antibodies)
RN  - 0 (Interleukin-6)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology/pharmacology
MH  - Blood-Brain Barrier/*pathology
MH  - Brain/blood supply/microbiology/*physiopathology
MH  - Cryptococcosis/microbiology/mortality/*pathology
MH  - Cryptococcus neoformans/growth & development/immunology
MH  - Humans
MH  - Interleukin-6/genetics/immunology/*pharmacology
MH  - Male
MH  - Meningitis, Fungal/microbiology/mortality/*pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Recombinant Proteins/genetics/pharmacology
EDAT- 2015/03/06 06:00
MHDA- 2015/12/30 06:00
CRDT- 2015/03/06 06:00
PHST- 2015/03/06 06:00 [entrez]
PHST- 2015/03/06 06:00 [pubmed]
PHST- 2015/12/30 06:00 [medline]
AID - 10.1007/s12038-014-9496-y [doi]
PST - ppublish
SO  - J Biosci. 2015 Mar;40(1):7-12. doi: 10.1007/s12038-014-9496-y.