PMID- 25740959
OWN - NLM
STAT- MEDLINE
DCOM- 20151014
LR  - 20181113
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 96
IP  - Pt 7
DP  - 2015 Jul
TI  - The predominant species of nonstructural protein 4B in hepatitis C 
      virus-replicating cells is not palmitoylated.
PG  - 1696-701
LID - 10.1099/vir.0.000111 [doi]
AB  - Hepatitis C virus (HCV) represents a significant global health burden. Viral 
      replication is thought to occur in close association with remodelled host cell 
      membranes, with non-structural protein 4B (NS4B) being a key player in this 
      process. NS4B is a poorly characterized integral membrane protein, which has been 
      reported to be palmitoylated at its carboxy-terminal end. In order to extend this 
      observation and to establish a functional role for NS4B palmitoylation, we sought 
      to determine the status of this post-translational modification when the protein 
      was expressed as part of a functional viral replicase. We performed direct 
      metabolic labelling and polyethylene glycol-maleimide palmitoylation reporter 
      assays on NS4B expressed in cells containing subgenomic replicons and infectious 
      viral RNA. In a vaccinia virus-based expression system NS4B palmitoylation was 
      detected in a genotype-dependent manner. However, in spite of the high 
      sensitivity of the methods used, no NS4B palmitoylation was found in 
      physiologically more relevant systems. Thus, NS4B palmitoylation is most likely 
      dispensable for HCV RNA replication.
FAU - Paul, David
AU  - Paul D
AD  - 1Department of Infectious Diseases, Molecular Virology, University of Heidelberg, 
      69120 Heidelberg, Germany.
FAU - Bartenschlager, Ralf
AU  - Bartenschlager R
AD  - 1Department of Infectious Diseases, Molecular Virology, University of Heidelberg, 
      69120 Heidelberg, Germany.
FAU - McCormick, Christopher
AU  - McCormick C
AD  - 2Faculty of Medicine and Institute for Life Science, University of Southampton, 
      Southampton SO16 6YD, UK.
LA  - eng
GR  - G0701215/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150304
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (NS4B protein, flavivirus)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Cell Line
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Hepacivirus/*physiology
MH  - Hepatocytes/virology
MH  - Humans
MH  - Lipoylation
MH  - *Protein Processing, Post-Translational
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Vaccinia virus/genetics
MH  - Viral Nonstructural Proteins/*chemistry/*metabolism
PMC - PMC4635453
EDAT- 2015/03/06 06:00
MHDA- 2015/10/16 06:00
PMCR- 2015/07/01
CRDT- 2015/03/06 06:00
PHST- 2015/03/06 06:00 [entrez]
PHST- 2015/03/06 06:00 [pubmed]
PHST- 2015/10/16 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
AID - vir.0.000111 [pii]
AID - 000111 [pii]
AID - 10.1099/vir.0.000111 [doi]
PST - ppublish
SO  - J Gen Virol. 2015 Jul;96(Pt 7):1696-701. doi: 10.1099/vir.0.000111. Epub 2015 Mar 
      4.