PMID- 25742289
OWN - NLM
STAT- MEDLINE
DCOM- 20160115
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 3
DP  - 2015
TI  - Detection of ROS1 gene rearrangement in lung adenocarcinoma: comparison of IHC, 
      FISH and real-time RT-PCR.
PG  - e0120422
LID - 10.1371/journal.pone.0120422 [doi]
LID - e0120422
AB  - AIMS: To compare fluorescence in situ hybridization (FISH), immunohistochemistry 
      (IHC) and quantitative real-time reverse transcription-PCR (qRT-PCR) assays for 
      detection of ROS1 fusion in a large number of ROS1-positive lung adenocatcinoma 
      (ADC) patients. METHODS: Using IHC analysis, sixty lung ADCs including 16 cases 
      with ROS1 protein expression and 44 cases without ROS1 expression were selected 
      for this study. The ROS1 fusion status was examined by FISH and qRT-PCR assay. 
      RESULTS: Among 60 cases, 16 (26.7%), 13 (21.7%) and 20 (33.3%) cases were ROS1 
      positive revealed by IHC, FISH and qRT-PCR, respectively. Using FISH as a 
      standard method for ROS1 fusion detection, the sensitivity and specificity of IHC 
      were 100% and 93.6%, respectively. Three IHC-positive cases, which showed FISH 
      negative, were demonstrated with ROS1 fusion by qRT-PCR analysis. The sensitivity 
      and specificity of qRT-PCR for detection for ROS1 fusion were 100% and 85.1%, 
      respectively. The total concordance rate between IHC and qRT-PCR were 93.3%. 
      CONCLUSION: IHC is a reliable and rapid screening tool in routine pathologic 
      laboratories for the identification of suitable candidates for ROS1-targeted 
      therapy. Some ROS1 IHC-positive but FISH-negative cases did harbor the 
      translocation events and may benefit from crizotinib.
FAU - Shan, Ling
AU  - Shan L
AD  - Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical 
      Sciences, Peking Union Medical College, Beijing, China.
FAU - Lian, Fang
AU  - Lian F
AD  - Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical 
      Sciences, Peking Union Medical College, Beijing, China.
FAU - Guo, Lei
AU  - Guo L
AD  - Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical 
      Sciences, Peking Union Medical College, Beijing, China.
FAU - Qiu, Tian
AU  - Qiu T
AD  - Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical 
      Sciences, Peking Union Medical College, Beijing, China.
FAU - Ling, Yun
AU  - Ling Y
AD  - Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical 
      Sciences, Peking Union Medical College, Beijing, China.
FAU - Ying, Jianming
AU  - Ying J
AD  - Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical 
      Sciences, Peking Union Medical College, Beijing, China.
FAU - Lin, Dongmei
AU  - Lin D
AD  - Department of Pathology, Cancer Institute & Hospital, Chinese Academy of Medical 
      Sciences, Peking Union Medical College, Beijing, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150305
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (ROS1 protein, human)
SB  - IM
MH  - Adenocarcinoma/*genetics/*metabolism
MH  - Adenocarcinoma of Lung
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms/*genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Oncogene Proteins, Fusion/genetics/metabolism
MH  - Protein-Tyrosine Kinases/genetics/metabolism
MH  - Proto-Oncogene Proteins/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
PMC - PMC4351102
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/03/06 06:00
MHDA- 2016/01/16 06:00
PMCR- 2015/03/05
CRDT- 2015/03/06 06:00
PHST- 2014/11/19 00:00 [received]
PHST- 2015/01/07 00:00 [accepted]
PHST- 2015/03/06 06:00 [entrez]
PHST- 2015/03/06 06:00 [pubmed]
PHST- 2016/01/16 06:00 [medline]
PHST- 2015/03/05 00:00 [pmc-release]
AID - PONE-D-14-50674 [pii]
AID - 10.1371/journal.pone.0120422 [doi]
PST - epublish
SO  - PLoS One. 2015 Mar 5;10(3):e0120422. doi: 10.1371/journal.pone.0120422. 
      eCollection 2015.