PMID- 25743476
OWN - NLM
STAT- MEDLINE
DCOM- 20151112
LR  - 20181202
IS  - 2049-632X (Electronic)
IS  - 2049-632X (Print)
IS  - 2049-632X (Linking)
VI  - 73
IP  - 3
DP  - 2015 Apr
TI  - Clostridium difficile toxin B intoxicated mouse colonic epithelial CT26 cells 
      stimulate the activation of dendritic cells.
LID - ftv008 [pii]
LID - 10.1093/femspd/ftv008 [doi]
AB  - Clostridium difficile causes antibiotic-associated diarrhea and pseudomembranous 
      colitis mainly through two exotoxins TcdA and TcdB that target intestinal 
      epithelial cells. Dendritic cells (DCs) play an important role in regulating 
      intestinal inflammatory responses. In the current study, we explored the 
      interaction of TcdB-intoxicated epithelial cells with mouse bone marrow-derived 
      DCs. TcdB induced cell death and heat shock protein translocation in mouse 
      intestinal epithelial CT26 cells. The intoxicated epithelial cells promoted the 
      phagocytosis and the TNF-alpha secretion by DCs. Incubation with TcdB-intoxicated 
      CT26 cells stimulated DC maturation. Moreover, TcdB-treated CT26 cells induced DC 
      immigration when they were injected into mice subcutaneously. Taken together, 
      these data demonstrate that TcdB-intoxicated intestinal epithelial cells are able 
      to stimulate DC activation in vitro and attract DCs in vivo, indicating that 
      epithelial cells may be able to regulate DC activation under the exposure of TcdB 
      during C. difficile infection.
CI  - (c) FEMS 2015. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Huang, Tuxiong
AU  - Huang T
AD  - School of Bioscience and Bioengineering, South China University of Technology 
      (SCUT), Guangzhou 51006, China Department of Microbial Pathogenesis, University 
      of Maryland Dental School, Baltimore, MD 21021, USA.
FAU - Perez-Cordon, Gregorio
AU  - Perez-Cordon G
AD  - Department of Microbial Pathogenesis, University of Maryland Dental School, 
      Baltimore, MD 21021, USA.
FAU - Shi, Lianfa
AU  - Shi L
AD  - Department of Microbial Pathogenesis, University of Maryland Dental School, 
      Baltimore, MD 21021, USA.
FAU - Li, Guangchao
AU  - Li G
AD  - School of Bioscience and Bioengineering, South China University of Technology 
      (SCUT), Guangzhou 51006, China.
FAU - Sun, Xingmin
AU  - Sun X
AD  - Department of Infectious Disease and Global Health, Tufts Cummings School of 
      Veterinary Medicine, North Grafton, MA 01536, USA.
FAU - Wang, Xiaoning
AU  - Wang X
AD  - Institute of Life Science, General Hospital of the People's Liberation Army, 
      Beijing 100853, China.
FAU - Wang, Jufang
AU  - Wang J
AD  - School of Bioscience and Bioengineering, South China University of Technology 
      (SCUT), Guangzhou 51006, China.
FAU - Feng, Hanping
AU  - Feng H
AD  - Department of Microbial Pathogenesis, University of Maryland Dental School, 
      Baltimore, MD 21021, USA hfeng@umaryland.edu.
LA  - eng
GR  - R01 AI088748/AI/NIAID NIH HHS/United States
GR  - R56 AI099458/AI/NIAID NIH HHS/United States
GR  - R01AI088748/AI/NIAID NIH HHS/United States
GR  - R01DK084509/DK/NIDDK NIH HHS/United States
GR  - U19 AI109776/AI/NIAID NIH HHS/United States
GR  - R01 DK084509/DK/NIDDK NIH HHS/United States
GR  - R56AI99458/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150127
PL  - United States
TA  - Pathog Dis
JT  - Pathogens and disease
JID - 101595366
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (toxB protein, Clostridium difficile)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/*toxicity
MH  - Bacterial Toxins/*toxicity
MH  - *Cell Communication
MH  - Cell Death/drug effects
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Dendritic Cells/*immunology/physiology
MH  - Epithelial Cells/*drug effects/*immunology/physiology
MH  - Heat-Shock Proteins/metabolism
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Phagocytosis
MH  - Protein Transport
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC4435672
MID - NIHMS689777
OTO - NOTNLM
OT  - Clostridium difficile
OT  - TcdB
OT  - dendritic cells
OT  - epithelial cells
OT  - inflammation
EDAT- 2015/03/07 06:00
MHDA- 2015/11/13 06:00
PMCR- 2016/04/01
CRDT- 2015/03/07 06:00
PHST- 2015/03/07 06:00 [entrez]
PHST- 2015/03/07 06:00 [pubmed]
PHST- 2015/11/13 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - ftv008 [pii]
AID - 10.1093/femspd/ftv008 [doi]
PST - ppublish
SO  - Pathog Dis. 2015 Apr;73(3):ftv008. doi: 10.1093/femspd/ftv008. Epub 2015 Jan 27.