PMID- 25744387
OWN - NLM
STAT- MEDLINE
DCOM- 20160216
LR  - 20191210
IS  - 1752-9867 (Electronic)
IS  - 1674-2052 (Linking)
VI  - 8
IP  - 3
DP  - 2015 Mar
TI  - Red-light-dependent interaction of phyB with SPA1 promotes COP1-SPA1 dissociation 
      and photomorphogenic development in Arabidopsis.
PG  - 467-78
LID - S1674-2052(14)00054-9 [pii]
LID - 10.1016/j.molp.2014.11.025 [doi]
AB  - Arabidopsis phytochromes (phyA-phyE) are photoreceptors dedicated to sensing 
      red/far-red light. Phytochromes promote photomorphogenic developments upon light 
      irradiation via a signaling pathway that involves rapid degradation of PIFs 
      (PHYTOCHROME INTERACTING FACTORS) and suppression of COP1 (CONSTITUTIVE 
      PHOTOMORPHOGENIC 1) nuclear accumulation, through physical interactions with PIFs 
      and COP1, respectively. Both phyA and phyB, the two best characterized 
      phytochromes, regulate plant photomorphogenesis predominantly under far-red light 
      and red light, respectively. It has been demonstrated that SPA1 (SUPPRESSOR OF 
      PHYTOCHROME A 1) associates with COP1 to promote COP1 activity and suppress 
      photomorphogenesis. Here, we report that the mechanism underlying phyB-promoted 
      photomorphogenesis in red light involves direct physical and functional 
      interactions between red-light-activated phyB and SPA1. We found that SPA1 acts 
      genetically downstream of PHYB to repress photomorphogenesis in red light. 
      Protein interaction studies in both yeast and Arabidopsis demonstrated that the 
      photoactivated phyB represses the association of SPA1 with COP1, which is 
      mediated, at least in part, through red-light-dependent interaction of phyB with 
      SPA1. Moreover, we show that phyA physically interacts with SPA1 in a 
      Pfr-form-dependent manner, and that SPA1 acts downstream of PHYA to regulate 
      photomorphogenesis in far-red light. This study provides a genetic and 
      biochemical model of how photoactivated phyB represses the activity of COP1-SPA1 
      complex through direct interaction with SPA1 to promote photomorphogenesis in red 
      light.
CI  - Copyright (c) 2015 The Author. Published by Elsevier Inc. All rights reserved.
FAU - Lu, Xue-Dan
AU  - Lu XD
AD  - Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of 
      Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
FAU - Zhou, Chuan-Miao
AU  - Zhou CM
AD  - National Key Laboratory of Plant Molecular Genetics (NKLPMG), Institute of Plant 
      Physiology and Ecology (SIPPE), Shanghai Institutes for Biological Sciences 
      (SIBS), Shanghai 200032, China.
FAU - Xu, Peng-Bo
AU  - Xu PB
AD  - School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Shanghai 
      200240, China.
FAU - Luo, Qian
AU  - Luo Q
AD  - Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of 
      Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
FAU - Lian, Hong-Li
AU  - Lian HL
AD  - Key Laboratory of Urban Agriculture (South) Ministry of Agriculture and School of 
      Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China. 
      Electronic address: hllian@sjtu.edu.cn.
FAU - Yang, Hong-Quan
AU  - Yang HQ
AD  - School of Life Sciences and Biotechnology, Shanghai Jiaotong University, Shanghai 
      200240, China. Electronic address: hqyang@sjtu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20141230
PL  - England
TA  - Mol Plant
JT  - Molecular plant
JID - 101465514
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (PHYB protein, Arabidopsis)
RN  - 0 (SPA1 protein, Arabidopsis)
RN  - 136250-22-1 (Phytochrome B)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Arabidopsis/genetics/*growth & development/*metabolism/radiation effects
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Plant
MH  - Light
MH  - Phytochrome B/genetics/*metabolism
MH  - Protein Binding/radiation effects
MH  - Ubiquitin-Protein Ligases
OTO - NOTNLM
OT  - COP1
OT  - SPA1
OT  - photoactivation
OT  - photomorphogenesis
OT  - phyA
OT  - phyB
EDAT- 2015/03/07 06:00
MHDA- 2016/02/18 06:00
CRDT- 2015/03/07 06:00
PHST- 2014/10/13 00:00 [received]
PHST- 2014/11/13 00:00 [revised]
PHST- 2014/11/14 00:00 [accepted]
PHST- 2015/03/07 06:00 [entrez]
PHST- 2015/03/07 06:00 [pubmed]
PHST- 2016/02/18 06:00 [medline]
AID - S1674-2052(14)00054-9 [pii]
AID - 10.1016/j.molp.2014.11.025 [doi]
PST - ppublish
SO  - Mol Plant. 2015 Mar;8(3):467-78. doi: 10.1016/j.molp.2014.11.025. Epub 2014 Dec 
      30.