PMID- 25744394
OWN - NLM
STAT- MEDLINE
DCOM- 20150609
LR  - 20220408
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 127
DP  - 2015 Apr 15
TI  - Involvement of TLR2 and TLR9 in the anti-inflammatory effects of chlorogenic acid 
      in HSV-1-infected microglia.
PG  - 12-8
LID - S0024-3205(15)00105-8 [pii]
LID - 10.1016/j.lfs.2015.01.036 [doi]
AB  - AIMS: There is no effective medication to date for herpes simplex virus 
      encephalitis (HSE). In this study, we investigated the anti-inflammatory effect 
      of chlorogenic acid (CGA) on herpes simplex virus (HSV)-1-induced responses in 
      BV2 microglia. MAIN METHODS: The cellular model was established with BV2 cells 
      stimulated by HSV-1 and then treated with CGA at different concentrations. Cell 
      viability was assayed by the MTT assay. The mRNA expression of Toll-like receptor 
      (TLR)-2, TLR9 and myeloid differentiation factor88 (Myd88) was assayed by 
      real-time quantitative PCR, and the protein expression was assayed by flow 
      cytometry or Western blotting. Tumor necrosis factor-alpha (TNF-alpha) and interleukin 
      (IL)-6 were measured by ELISA as well as real-time quantitative PCR. Nuclear 
      NF-kappaB p65 protein was assayed by Western blotting. KEY FINDINGS: The cell 
      survival rate was significantly improved after CGA treatment, and CGA prevented 
      increases in TLR2, TLR9 and Myd88 following HSV-1 challenge in BV2 cells both at 
      the mRNA and protein levels. Moreover, CGA could attenuate HSV-induced TNF-alpha and 
      IL-6 release into the supernatant. The mRNA levels of TNF-alpha and IL-6 were also 
      significantly inhibited by CGA. The expression of NF-kappaB p65 increased 
      significantly in the nucleus in HSV-1-stimulated microglia but could be reduced 
      by CGA. SIGNIFICANCE: CGA inhibits the inflammatory reaction in HSE via the 
      suppression of TLR2/TLR9-Myd88 signaling pathways. CGA may serve as an 
      anti-inflammatory agent and provide a new strategy for treating HSE.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Guo, Yuan-Jin
AU  - Guo YJ
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, PR China.
FAU - Luo, Tao
AU  - Luo T
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, PR China; Department of 
      Neurology, Wuhan Central Hospital, Wuhan 430014, PR China.
FAU - Wu, Fei
AU  - Wu F
AD  - Department of Neurology, Wuhan General Hospital of Guangzhou Military Command, 
      Wuhan 430070, PR China.
FAU - Mei, Yuan-Wu
AU  - Mei YW
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, PR China.
FAU - Peng, Jun
AU  - Peng J
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, PR China.
FAU - Liu, Huan
AU  - Liu H
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, PR China.
FAU - Li, Hua-Rong
AU  - Li HR
AD  - Department of Infectious Diseases, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430022, PR China.
FAU - Zhang, Shu-Ling
AU  - Zhang SL
AD  - Department of Infectious Diseases, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430022, PR China.
FAU - Dong, Ji-Hua
AU  - Dong JH
AD  - Central Lab, Union Hospital, Tongji Medical College, Huazhong University of 
      Science and Technology, Wuhan 430022, PR China.
FAU - Fang, Yuan
AU  - Fang Y
AD  - Department of Neurology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, PR China.
FAU - Zhao, Lei
AU  - Zhao L
AD  - Department of Infectious Diseases, Union Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430022, PR China. Electronic 
      address: leizhao@hust.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150302
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 9)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 318ADP12RI (Chlorogenic Acid)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Chlorogenic Acid/*pharmacology
MH  - Cytokines/metabolism
MH  - Herpes Simplex/*pathology/virology
MH  - *Herpesvirus 1, Human
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Microglia/*drug effects
MH  - NF-kappa B/drug effects/metabolism
MH  - Signal Transduction/drug effects
MH  - Toll-Like Receptor 2/biosynthesis/*drug effects
MH  - Toll-Like Receptor 9/biosynthesis/*drug effects
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Chlorogenic acid
OT  - Herpes simplex virus-1
OT  - Inflammation
OT  - Toll-like receptor 2
OT  - Toll-like receptor 9
EDAT- 2015/03/10 06:00
MHDA- 2015/06/10 06:00
CRDT- 2015/03/07 06:00
PHST- 2014/08/11 00:00 [received]
PHST- 2015/01/29 00:00 [revised]
PHST- 2015/01/30 00:00 [accepted]
PHST- 2015/03/07 06:00 [entrez]
PHST- 2015/03/10 06:00 [pubmed]
PHST- 2015/06/10 06:00 [medline]
AID - S0024-3205(15)00105-8 [pii]
AID - 10.1016/j.lfs.2015.01.036 [doi]
PST - ppublish
SO  - Life Sci. 2015 Apr 15;127:12-8. doi: 10.1016/j.lfs.2015.01.036. Epub 2015 Mar 2.