PMID- 25744604
OWN - NLM
STAT- MEDLINE
DCOM- 20160104
LR  - 20150324
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 25
IP  - 2
DP  - 2015 Apr
TI  - Tp17 membrane protein of Treponema pallidum activates endothelial cells in vitro.
PG  - 538-44
LID - S1567-5769(15)00084-3 [pii]
LID - 10.1016/j.intimp.2015.02.028 [doi]
AB  - Tp17, a membrane immunogen of Treponema pallidum subsp. pallidum, was initially 
      recognized as an inflammatory mediator of syphilis. Because the histopathology of 
      syphilis is characterized by endothelial cell abnormalities, we investigated the 
      effects of recombinant Tp17 (rTp17) on endothelial cell activation in vitro. 
      Using real-time reverse transcription-PCR and whole-cell ELISA, we found that 
      rTp17 activated endothelial cells, as demonstrated by the up-regulated expression 
      and increased gene transcription of intercellular adhesion molecule 1 (ICAM-1), 
      E-selectin, and monocyte chemoattractant protein-1 (MCP-1). rTp17 also enhanced 
      the migration and subsequent adhesion of monocytes to endothelial cells as well 
      as increased transendothelial migration of monocytes. These data suggest that the 
      ability of Tp17 to activate endothelial cells may play an important role in the 
      immunopathogenesis of syphilis.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Zhang, Rui-Li
AU  - Zhang RL
AD  - Department of Dermatology, Wuxi Second Affiliated Hospital of Nanjing Medical 
      University, Wuxi, Jiangsu Province, China.
FAU - Wang, Qian-Qiu
AU  - Wang QQ
AD  - Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing, Jiangsu 
      Province, China; Peking Union Medical College, Nanjing, Jiangsu Province, China; 
      National Center for STD Control, China Centers for Diseases Control and 
      Prevention, Nanjing, Jiangsu Province, China. Electronic address: 
      doctorwqq@163.com.
FAU - Zhang, Jing-Ping
AU  - Zhang JP
AD  - Institute of Dermatology, Chinese Academy of Medical Sciences, Nanjing, Jiangsu 
      Province, China; Peking Union Medical College, Nanjing, Jiangsu Province, China; 
      National Center for STD Control, China Centers for Diseases Control and 
      Prevention, Nanjing, Jiangsu Province, China.
FAU - Yang, Li-Jia
AU  - Yang LJ
AD  - Department of Dermatology, Wuxi Second Affiliated Hospital of Nanjing Medical 
      University, Wuxi, Jiangsu Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150302
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Proteins)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (E-Selectin)
RN  - 0 (Lipoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 148068-13-7 (17 kDa immunogen, Treponema pallidum)
SB  - IM
MH  - Antigens, Bacterial/*pharmacology
MH  - Bacterial Proteins/*pharmacology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics
MH  - E-Selectin/genetics
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - Lipoproteins/*pharmacology
MH  - Membrane Proteins/*pharmacology
MH  - Recombinant Proteins/pharmacology
OTO - NOTNLM
OT  - Activation
OT  - Endothelial cells
OT  - Tp17 protein
OT  - Treponema pallidum
EDAT- 2015/03/07 06:00
MHDA- 2016/01/05 06:00
CRDT- 2015/03/07 06:00
PHST- 2014/11/26 00:00 [received]
PHST- 2015/02/12 00:00 [revised]
PHST- 2015/02/18 00:00 [accepted]
PHST- 2015/03/07 06:00 [entrez]
PHST- 2015/03/07 06:00 [pubmed]
PHST- 2016/01/05 06:00 [medline]
AID - S1567-5769(15)00084-3 [pii]
AID - 10.1016/j.intimp.2015.02.028 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2015 Apr;25(2):538-44. doi: 10.1016/j.intimp.2015.02.028. 
      Epub 2015 Mar 2.