PMID- 25744689
OWN - NLM
STAT- MEDLINE
DCOM- 20151117
LR  - 20181202
IS  - 1361-6528 (Electronic)
IS  - 0957-4484 (Linking)
VI  - 26
IP  - 12
DP  - 2015 Mar 27
TI  - Effect of HSA coated iron oxide labeling on human umbilical cord derived 
      mesenchymal stem cells.
PG  - 125103
LID - 10.1088/0957-4484/26/12/125103 [doi]
AB  - Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) are known for 
      self-renewal and differentiation into cells of various lineages like bone, 
      cartilage and fat. They have been used in biomedical applications to treat 
      degenerative disorders. However, to exploit the therapeutic potential of stem 
      cells, there is a requirement of sensitive non-invasive imaging techniques which 
      will offer the ability to track transplanted cells, bio-distribution, 
      proliferation and differentiation. In this study, we have analyzed the efficacy 
      of human serum albumin coated iron oxide nanoparticles (HSA-IONPs) on the 
      differentiation of hUC-MSCs. The colloidal stability of the HSA-IONPs was tested 
      over a long period of time (>/=20 months) and the optimized concentration of 
      HSA-IONPs for labeling the stem cells was 60 mug ml(-1). Detailed in vitro assays 
      have been performed to ascertain the effect of the nanoparticles (NPs) on stem 
      cells. Lactate dehydrogenase (LDH) assay showed minimum release of LDH depicting 
      the least disruptions in cellular membrane. At the same time, mitochondrial 
      impairment of the cells was also not observed by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Flow 
      cytometry analysis revealed lesser generation of reactive oxygen species in 
      HSA-IONPs labeled hUC-MSCs in comparison to bare and commercial IONPs. 
      Transmission electron microscopy showed endocytic engulfment of the NPs by the 
      hUC-MSCs. During the process, the gross morphologies of the actin cytoskeleton 
      were found to be intact as shown by immunofluorescence microscopy. Also, the 
      engulfment of the HSA-IONPs did not show any detrimental effect on the 
      differentiation potential of the stem cells into adipocytes, osteocytes and 
      chondrocytes, thereby confirming that the inherent properties of stem cells were 
      maintained.
FAU - Sanganeria, Purva
AU  - Sanganeria P
AD  - Department of Biological Sciences, School of Science, NMIMS University, Vile 
      Parle (West), Mumbai 400056, India.
FAU - Chandra, Sudeshna
AU  - Chandra S
FAU - Bahadur, Dhirendra
AU  - Bahadur D
FAU - Khanna, Aparna
AU  - Khanna A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150306
PL  - England
TA  - Nanotechnology
JT  - Nanotechnology
JID - 101241272
RN  - 0 (Contrast Media)
RN  - 0 (Ferric Compounds)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Serum Albumin)
RN  - 1K09F3G675 (ferric oxide)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Contrast Media/adverse effects/pharmacokinetics/*pharmacology
MH  - Cytoskeleton/drug effects
MH  - Ferric Compounds/*chemistry
MH  - Humans
MH  - Mesenchymal Stem Cells/drug effects/*physiology/ultrastructure
MH  - Nanoparticles/*chemistry
MH  - Reactive Oxygen Species
MH  - Serum Albumin/*chemistry
MH  - Umbilical Cord/cytology
EDAT- 2015/03/07 06:00
MHDA- 2015/11/18 06:00
CRDT- 2015/03/07 06:00
PHST- 2015/03/07 06:00 [entrez]
PHST- 2015/03/07 06:00 [pubmed]
PHST- 2015/11/18 06:00 [medline]
AID - 10.1088/0957-4484/26/12/125103 [doi]
PST - ppublish
SO  - Nanotechnology. 2015 Mar 27;26(12):125103. doi: 10.1088/0957-4484/26/12/125103. 
      Epub 2015 Mar 6.