PMID- 25744840
OWN - NLM
STAT- MEDLINE
DCOM- 20150702
LR  - 20150306
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 31
IP  - 3
DP  - 2015 Mar
TI  - [Inhibitory effect of baicalein on the proliferation and invasion of osteosarcoma 
      cells and mechanism].
PG  - 342-5
AB  - OBJECTIVE: To explore the effect of baicalein on the proliferation and invasion 
      of osteosarcoma cells and its related mechanism. METHODS: Osteosarcoma MG-63 
      cells that were cultured in vitro were respectively treated with 20 muL culture 
      medium (control group), dehydrated alcohol (0 mumol/L baicalein group), 100 and 
      200 mumol/L baicalein solution for 48 hours. Cell proliferation was analyzed by 
      MTT assay. The cell invasion ability was detected using Transwell(TM) invasion 
      assay. The expression of ezrin mRNA was examined by real-time quantitative PCR. 
      The expressions of ezrin protein and p-ezrin protein were measured using Western 
      blotting. Apoptosis index (AI) was determined by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL). RESULTS: The inhibitory 
      rates of cell proliferation significantly increased in 100 and 200 mumol/L 
      baicalein groups as compared with 0 mumol/L baicalein group. Moreover, that was 
      higher in 200 mumol/L baicalein group than in 100 mumol/L baicalein group. In 
      comparison with control and 0 mumol/L baicalein groups, the mean cell numbers of 
      permeated membrane and levels of ezrin mRNA, ezrin protein and p-ezrin protein 
      gradually decreased, but AI was gradually elevated with the increase of baicalein 
      concentrations, whereas there was no significant difference in these indicators 
      between 0 mumol/L baicalein group and control group. CONCLUSION: Baicalein can 
      inhibit the proliferation and invasion of osteosarcoma MG-63 cells. The mechanism 
      may be associated with the inhibited expression and activity of ezrin protein and 
      the promoted tumor cell apoptosis.
FAU - Tang, Zhibin
AU  - Tang Z
AD  - Department of Orthopedics, First Affiliated Hospital, University of South China, 
      Hengyang 421001, China.
FAU - Li, Chun
AU  - Li C
AD  - Department of Orthopedics, First Affiliated Hospital, University of South China, 
      Hengyang 421001, China.
FAU - Chen, Zhiwei
AU  - Chen Z
AD  - Department of Orthopedics, First Affiliated Hospital, University of South China, 
      Hengyang 421001, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavanones)
RN  - 0 (ezrin)
RN  - 49QAH60606 (baicalein)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Cytoskeletal Proteins/genetics/metabolism
MH  - Down-Regulation/drug effects
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Flavanones/*pharmacology
MH  - Humans
MH  - Neoplasm Invasiveness
MH  - Osteosarcoma/drug therapy/genetics/metabolism/*physiopathology
EDAT- 2015/03/07 06:00
MHDA- 2015/07/03 06:00
CRDT- 2015/03/07 06:00
PHST- 2015/03/07 06:00 [entrez]
PHST- 2015/03/07 06:00 [pubmed]
PHST- 2015/07/03 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Mar;31(3):342-5.