PMID- 25746371
OWN - NLM
STAT- MEDLINE
DCOM- 20160104
LR  - 20150324
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 25
IP  - 2
DP  - 2015 Apr
TI  - Anti-allergic activity of R-phycocyanin from Porphyra haitanensis in 
      antigen-sensitized mice and mast cells.
PG  - 465-73
LID - S1567-5769(15)00088-0 [pii]
LID - 10.1016/j.intimp.2015.02.032 [doi]
AB  - The prevalence of food allergy has increased in Asian countries. Marine algae 
      have been proposed as the potential resource for anti-allergic therapeutics. The 
      present study was aimed at isolating R-phycocyanin (RPC) from Porphyra 
      haitanensis and determining the anti-allergy potential of RPC in 
      antigen-sensitized mice and mast cells. In animal experiments, RPC could 
      effectively reduce tropomyosin (TM)-specific immunoglobulin E (IgE) and histamine 
      levels, alleviate allergy symptoms and jejunum tissue inflammation in mice, and 
      inhibit the expression and release of cytokines (interleukin-4 (IL-4) and IL-13) 
      in peritoneal lavage fluid. In spleen lymphocyte experiments, high purity of RPC 
      skewed the immunological function of CD4(+) T cells towards Th1 activity. A 
      higher expression of interferon (IFN)-gamma was induced by a synergistic effect of TM 
      and RPC. Through the Jun N-terminal kinase and Janus kinase 2 signaling pathways, 
      IFN-gamma synthesis was induced by RPC in combination with TM. Anti-allergic effect 
      of RPC was evaluated in IgE-mediated rat mast RBL-2H3 cells. The results 
      demonstrated that RPC inhibited allergy markers, including the release of 
      beta-hexosaminidase, histamine and ROS in antigen-sensitized RBL-2H3 cells. RPC also 
      suppressed the production of pro-inflammatory factors (IL-4 and tumor necrosis 
      factor-alpha). In conclusion, RPC decreased allergic sensitization against TM by 
      blocking Th2 cell polarization as well as suppressed the release of 
      allergic-mediators in antigen-stimulated mast cells. It may be used as a 
      functional food component or active pharmaceutical ingredient for allergic 
      patients.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Liu, Qingmei
AU  - Liu Q
AD  - College of Food and Biological Engineering, Fujian Collaborative Innovation 
      Center for Exploitation and Utilization of Marine Biological Resources, Jimei 
      University, 43 Yindou Road, Xiamen, 361021 Fujian, PR China.
FAU - Wang, Youzhao
AU  - Wang Y
AD  - College of Food and Biological Engineering, Fujian Collaborative Innovation 
      Center for Exploitation and Utilization of Marine Biological Resources, Jimei 
      University, 43 Yindou Road, Xiamen, 361021 Fujian, PR China.
FAU - Cao, Minjie
AU  - Cao M
AD  - College of Food and Biological Engineering, Fujian Collaborative Innovation 
      Center for Exploitation and Utilization of Marine Biological Resources, Jimei 
      University, 43 Yindou Road, Xiamen, 361021 Fujian, PR China.
FAU - Pan, Tzuming
AU  - Pan T
AD  - Department of Biochemical Science and Technology, College of Life Science, 
      National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
FAU - Yang, Yang
AU  - Yang Y
AD  - College of Food and Biological Engineering, Fujian Collaborative Innovation 
      Center for Exploitation and Utilization of Marine Biological Resources, Jimei 
      University, 43 Yindou Road, Xiamen, 361021 Fujian, PR China.
FAU - Mao, Haiyan
AU  - Mao H
AD  - College of Food and Biological Engineering, Fujian Collaborative Innovation 
      Center for Exploitation and Utilization of Marine Biological Resources, Jimei 
      University, 43 Yindou Road, Xiamen, 361021 Fujian, PR China.
FAU - Sun, Lechang
AU  - Sun L
AD  - College of Food and Biological Engineering, Fujian Collaborative Innovation 
      Center for Exploitation and Utilization of Marine Biological Resources, Jimei 
      University, 43 Yindou Road, Xiamen, 361021 Fujian, PR China.
FAU - Liu, Guangming
AU  - Liu G
AD  - College of Food and Biological Engineering, Fujian Collaborative Innovation 
      Center for Exploitation and Utilization of Marine Biological Resources, Jimei 
      University, 43 Yindou Road, Xiamen, 361021 Fujian, PR China. Electronic address: 
      gmliu@jmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150304
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Tropomyosin)
RN  - 11016-15-2 (Phycocyanin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Anti-Allergic Agents/*pharmacology/*therapeutic use
MH  - Antigens/immunology
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cytokines/immunology
MH  - Feces/chemistry
MH  - Female
MH  - Histamine/blood/immunology
MH  - Hypersensitivity/blood/*drug therapy/immunology
MH  - Immunoglobulin E/blood
MH  - Jejunum/immunology
MH  - Mast Cells/*drug effects/immunology
MH  - Mice, Inbred BALB C
MH  - Phycocyanin/*pharmacology/*therapeutic use
MH  - Porphyra
MH  - Rats
MH  - Th2 Cells/immunology
MH  - Tropomyosin/immunology
OTO - NOTNLM
OT  - Anti-allergic activity
OT  - Murine model
OT  - Porphyra haitanensis
OT  - R-phycocyanin
OT  - RBL-2H3 cell
OT  - Th2 immune response
EDAT- 2015/03/10 06:00
MHDA- 2016/01/05 06:00
CRDT- 2015/03/10 06:00
PHST- 2014/12/24 00:00 [received]
PHST- 2015/02/19 00:00 [revised]
PHST- 2015/02/20 00:00 [accepted]
PHST- 2015/03/10 06:00 [entrez]
PHST- 2015/03/10 06:00 [pubmed]
PHST- 2016/01/05 06:00 [medline]
AID - S1567-5769(15)00088-0 [pii]
AID - 10.1016/j.intimp.2015.02.032 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2015 Apr;25(2):465-73. doi: 10.1016/j.intimp.2015.02.032. 
      Epub 2015 Mar 4.