PMID- 25747042
OWN - NLM
STAT- MEDLINE
DCOM- 20151023
LR  - 20160203
IS  - 1880-5779 (Electronic)
IS  - 1341-7568 (Linking)
VI  - 89
IP  - 4
DP  - 2014
TI  - Hyperexpansion of wheat chromosomes sorted by flow cytometry.
PG  - 181-5
LID - 10.1266/ggs.89.181 [doi]
AB  - Despite remarkable recent progress in the analysis of plant genome organization 
      and chromosome structure, there is a need for methods enabling DNA sequences to 
      be mapped by fluorescence in situ hybridization (FISH) at high spatial 
      resolution. We sorted mitotic metaphase chromosomes of wheat by flow cytometry 
      and observed the occurrence of hyperexpanded chromosomes among them. However, 
      this phenomenon was not reproducible in subsequent experiments. An investigation 
      into the procedures of flow cytometry revealed that the hyperexpansion of 
      chromosomes became reproducible when the concentration of formaldehyde used in 
      sample fixation was reduced. We conducted FISH analysis with 45S rDNA, 5S rDNA 
      and wheat centromeric repeat sequences as probes on flow-sorted chromosomes and 
      also on chromosomes from squash preparations. We measured the length of 
      chromosomes 1B and 6B, identified by FISH. On average, the hyperexpanded 1B and 
      6B chromosomes were 7.26 and 7.53 times longer, respectively, than the same 
      chromosomes from the squash preparations. The most stretched 1B and 6B 
      chromosomes both exceeded 100 micrometers.
FAU - Endo, Takashi R
AU  - Endo TR
AD  - Institute of Experimental Botany, Centre of the Region Hana for Biotechnological 
      and Agricultural Research, Slechtitelu 31; Palacky University Olomouc, Centre of 
      the Region Hana for Biotechnological and Agricultural Research,11, 783 71 Czech 
      Republic. endo@ueb.cas.cz
FAU - Kubalakova, Marie
AU  - Kubalakova M
FAU - Vrana, Jan
AU  - Vrana J
FAU - Dolezel, Jaroslav
AU  - Dolezel J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Genes Genet Syst
JT  - Genes & genetic systems
JID - 9607822
SB  - IM
MH  - Chromosomes, Plant/genetics/*ultrastructure
MH  - Flow Cytometry
MH  - In Situ Hybridization, Fluorescence
MH  - Triticum/*genetics
EDAT- 2014/01/01 00:00
MHDA- 2015/10/24 06:00
CRDT- 2015/03/10 06:00
PHST- 2015/03/10 06:00 [entrez]
PHST- 2014/01/01 00:00 [pubmed]
PHST- 2015/10/24 06:00 [medline]
AID - 10.1266/ggs.89.181 [doi]
PST - ppublish
SO  - Genes Genet Syst. 2014;89(4):181-5. doi: 10.1266/ggs.89.181.