PMID- 25748337
OWN - NLM
STAT- MEDLINE
DCOM- 20150723
LR  - 20150330
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 33
IP  - 16
DP  - 2015 Apr 15
TI  - Pleurotus ferulae water extract enhances the maturation and function of murine 
      bone marrow-derived dendritic cells through TLR4 signaling pathway.
PG  - 1923-33
LID - S0264-410X(15)00263-7 [pii]
LID - 10.1016/j.vaccine.2015.02.063 [doi]
AB  - Dendritic cells (DCs) play important roles in the regulation of immune system, 
      which link innate and adaptive immune responses. Mature DCs produced interleukin 
      (IL)-12 promote optimal type 1 T helper (Th1) cells and cytotoxic T lymphocytes. 
      The extracts of traditional herbal medicines have been shown to enhance immune 
      responses through promoting the maturation and cytokine production of DCs. Here, 
      we investigated the effects of Pleurotus ferulae water extract (PFWE) on the 
      maturation and function of bone marrow-derived DCs (BM-DCs). Upon PFWE treatment, 
      BM-DCs dose-dependently upregulated the expression of CD40, CD80, CD86 and MHC II 
      and increased the production of IL-12, IL-6 and tumor necrosis factor (TNF)-alpha but 
      not for IL-10, which is mediated by TLR4 signaling pathway, at least partially. 
      The production of prostaglandin E2 (PGE2) in BM-DCs was decreased by the 
      treatment of PFWE. Moreover, PFWE treatment decreased the expression of active 
      caspase-3 but increased the expression of CCR7. PFWE treated DCs enhanced the 
      proliferation of allogenic CD8(+) T cells and the capacity of antigen presenting 
      to autologous CD8(+) T cells. The combination of PFWE and CpG-ODN further 
      enhanced the maturation and function of murine BM-DCs. The results showed that 
      PFWE could enhance the maturation and function of DCs through TLR4 signaling 
      pathway and has additive effect when combined with CpG-ODN, suggesting that PFWE 
      alone or combined with CpG-ODN could be used to enhance the immune responses.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Li, Jinyao
AU  - Li J
AD  - College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 
      Urumqi 830046, Xinjiang, China. Electronic address: ljyxju@qq.com.
FAU - Wang, Xinhui
AU  - Wang X
AD  - College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 
      Urumqi 830046, Xinjiang, China.
FAU - Wang, Weilan
AU  - Wang W
AD  - College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 
      Urumqi 830046, Xinjiang, China.
FAU - Luo, JiaoJiao
AU  - Luo J
AD  - College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 
      Urumqi 830046, Xinjiang, China.
FAU - Aipire, Adila
AU  - Aipire A
AD  - College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 
      Urumqi 830046, Xinjiang, China.
FAU - Li, Jinyu
AU  - Li J
AD  - College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 
      Urumqi 830046, Xinjiang, China.
FAU - Zhang, Fuchun
AU  - Zhang F
AD  - College of Life Science and Technology, Xinjiang University, 14 Shengli Road, 
      Urumqi 830046, Xinjiang, China. Electronic address: zfcxju@qq.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150305
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Biological Products)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Toll-Like Receptor 4)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Apoptosis/drug effects
MH  - Biological Products/*pharmacology
MH  - Bone Marrow Cells/cytology/*drug effects/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/cytology/*drug effects/*metabolism
MH  - Dinoprostone/biosynthesis
MH  - Female
MH  - Immunophenotyping
MH  - Inflammation Mediators/metabolism
MH  - Mice
MH  - Phenotype
MH  - Pleurotus/*chemistry
MH  - *Signal Transduction
MH  - T-Lymphocyte Subsets/immunology/metabolism
MH  - Toll-Like Receptor 4/*metabolism
OTO - NOTNLM
OT  - CpG-ODN
OT  - Cytokine production
OT  - Dendritic cell
OT  - Maturation
OT  - Pleurotus ferulae water extract
EDAT- 2015/03/10 06:00
MHDA- 2015/07/24 06:00
CRDT- 2015/03/10 06:00
PHST- 2014/09/25 00:00 [received]
PHST- 2015/01/29 00:00 [revised]
PHST- 2015/02/24 00:00 [accepted]
PHST- 2015/03/10 06:00 [entrez]
PHST- 2015/03/10 06:00 [pubmed]
PHST- 2015/07/24 06:00 [medline]
AID - S0264-410X(15)00263-7 [pii]
AID - 10.1016/j.vaccine.2015.02.063 [doi]
PST - ppublish
SO  - Vaccine. 2015 Apr 15;33(16):1923-33. doi: 10.1016/j.vaccine.2015.02.063. Epub 
      2015 Mar 5.