PMID- 2575063
OWN - NLM
STAT- MEDLINE
DCOM- 19900214
LR  - 20190912
IS  - 0435-1339 (Print)
IS  - 0435-1339 (Linking)
VI  - 24
IP  - 6
DP  - 1989 Dec
TI  - Somatostatin receptors and the effect of somatostatin on histamine-stimulated 
      adenylate cyclase activity in isolated gastric glands of guinea pigs.
PG  - 611-8
AB  - To investigate the mechanism of the suppressive action of somatostatin on gastric 
      acid secretion, we determined the somatostatin binding and adenylate cyclase 
      activity on crude membrane fractions of isolated gastric glands of guinea pigs. 
      The binding studies using 125I-Tyr11 somatostatin showed the presence of 
      somatostatin receptors with a single high affinity on crude membrane fractions. 
      The dissociation constant (KD) for somatostatin was 1.05 +/- 0.13 nM, and the 
      number of binding sites (Bmax) was 6.98 +/- 1.27 fmol/mg protein (mean +/- SE, n 
      = 6). The adenylate cyclase activity was increased by histamine, which was 
      completely inhibited by 10(-3) M cimetidine. Somatostatin non-competitively 
      suppressed the histamine-stimulated adenylate cyclase activity in the presence of 
      guanosine 5'-triphosphate (GTP). These results suggest that somatostatin inhibits 
      histamine-stimulated acid secretion through the inhibition of the adenylate 
      cyclase system, via somatostatin receptor and guanine nucleotide binding protein, 
      which is activated by GTP binding.
FAU - Yoshida, K
AU  - Yoshida K
AD  - Third Department of Internal Medicine, Kyushu University Faculty of Medicine, 
      Fukuoka, Japan.
FAU - Nishihara, S
AU  - Nishihara S
FAU - Misawa, T
AU  - Misawa T
FAU - Nawata, H
AU  - Nawata H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Gastroenterol Jpn
JT  - Gastroenterologia Japonica
JID - 0152744
RN  - 0 (Receptors, Neurotransmitter)
RN  - 0 (Receptors, Somatostatin)
RN  - 51110-01-1 (Somatostatin)
RN  - 59481-27-5 (somatostatin, Tyr(11)-)
RN  - 820484N8I3 (Histamine)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/*metabolism
MH  - Animals
MH  - Gastric Acid/*metabolism
MH  - Gastric Mucosa/*drug effects/enzymology/metabolism
MH  - Guinea Pigs
MH  - Histamine/*pharmacology
MH  - In Vitro Techniques
MH  - Male
MH  - Receptors, Neurotransmitter/*drug effects/metabolism
MH  - Receptors, Somatostatin
MH  - Somatostatin/*analogs & derivatives/metabolism/pharmacology
EDAT- 1989/12/01 00:00
MHDA- 1989/12/01 00:01
CRDT- 1989/12/01 00:00
PHST- 1989/12/01 00:00 [pubmed]
PHST- 1989/12/01 00:01 [medline]
PHST- 1989/12/01 00:00 [entrez]
AID - 10.1007/BF02774158 [doi]
PST - ppublish
SO  - Gastroenterol Jpn. 1989 Dec;24(6):611-8. doi: 10.1007/BF02774158.