PMID- 25751103
OWN - NLM
STAT- MEDLINE
DCOM- 20150930
LR  - 20150509
IS  - 1945-7197 (Electronic)
IS  - 0021-972X (Linking)
VI  - 100
IP  - 5
DP  - 2015 May
TI  - Effect of short-term hyperglycemia on adipose tissue fluxes of selected cytokines 
      in vivo during multiple phases of diet-induced weight loss in obese women.
PG  - 1949-56
LID - 10.1210/jc.2014-3846 [doi]
AB  - CONTEXT: Hyperglycemia is suggested to be one of the drivers of the 
      proinflammatory state observed in obese and diabetic patients. OBJECTIVES: The 
      objectives of the study was to investigate whether sc abdominal adipose tissue 
      (scAT) could be one of the important sources of proinflammatory cytokines 
      released in response to short-term hyperglycemia and whether this secretion 
      capacity could be influenced by weight loss. DESIGN, PATIENTS, AND INTERVENTIONS: 
      Output of cytokines and proteins of acute phase from scAT in response to a 
      3-hours hyperglycemic clamp was evaluated in nine obese women in vivo using 
      Fick's principle. Moreover, the output of cytokines was analyzed during a 
      multiphase dietary intervention consisting of 1 month on a very low-calorie diet 
      and subsequent 5-month weight-stabilization phase. MAIN OUTCOME MEASURE(S): The 
      levels of cytokines and proteins of acute phase [IL-6, IL-8, IL-1 receptor 
      antagonist (IL-1Ra), TNF-alpha, monocyte chemoattractant protein-1 (MCP-1), serum 
      amyloid A, and C-reactive protein] in arterial and venous plasma were measured 
      during each dietary phase. The insulin sensitivity of scAT in respect to the 
      antilipolytic effect of insulin during the clamp was assessed. RESULTS: 
      Hyperglycemia increased the output of cytokines IL-6, MCP-1, and IL-1Ra from 
      scAT. This effect had a tendency to be reduced after weight loss. The output of 
      other proinflammatory substances from scAT into circulation was not detected. The 
      diet-induced weight loss was associated with the improvement of antilipolytic 
      insulin sensitivity in scAT. CONCLUSIONS: The results suggest that short-term 
      hyperglycemia induces an increase of the output of cytokines IL-6, IL-1Ra, and 
      MCP-1 from adipose tissue, and this deleterious hyperglycemia effect may be 
      attenuated by the diet-induced weight reduction. This lowered responsiveness of 
      the inflammation-related system may be an important feature of the weight 
      reduction-induced improvement of the metabolic status of obese prediabetic 
      individuals.
FAU - Siklova, Michaela
AU  - Siklova M
AD  - Department of Sports Medicine (M.S., J.P., V.S.), Third Faculty of Medicine, 
      Charles University, 100 00 Prague, Czech Republic; Department of Clinical 
      Physiology (L.S., J.B.), Bispebjerg University Hospital, 2400 Copenhagen, 
      Denmark; and Department of Biomedical Sciences (J.B.), University of Copenhagen, 
      DK-1165 Copenhagen, Denmark.
FAU - Simonsen, Lene
AU  - Simonsen L
FAU - Polak, Jan
AU  - Polak J
FAU - Stich, Vladimir
AU  - Stich V
FAU - Bulow, Jens
AU  - Bulow J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150309
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Cytokines)
SB  - IM
MH  - Adipose Tissue/*metabolism
MH  - Adult
MH  - Cytokines/*blood
MH  - Diet, Reducing
MH  - Female
MH  - Humans
MH  - Hyperglycemia/*metabolism
MH  - Obesity/diet therapy/*metabolism
MH  - Weight Loss/*physiology
EDAT- 2015/03/10 06:00
MHDA- 2015/10/01 06:00
CRDT- 2015/03/10 06:00
PHST- 2015/03/10 06:00 [entrez]
PHST- 2015/03/10 06:00 [pubmed]
PHST- 2015/10/01 06:00 [medline]
AID - 10.1210/jc.2014-3846 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2015 May;100(5):1949-56. doi: 10.1210/jc.2014-3846. Epub 
      2015 Mar 9.