PMID- 25751856
OWN - NLM
STAT- MEDLINE
DCOM- 20150925
LR  - 20150310
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 113
IP  - 2
DP  - 2015 Mar 9
TI  - Experimental infections of Pacific oyster Crassostrea gigas using the Australian 
      ostreid herpesvirus-1 (OsHV-1) microVar strain.
PG  - 137-47
LID - 10.3354/dao02826 [doi]
AB  - In Australia, the spread of the ostreid herpesvirus-1 microvariant (OsHV-1 microVar) 
      threatens the Pacific oyster industry. There is an urgent need to develop an 
      experimental infection model in order to study the pathogenesis of the virus 
      under controlled laboratory conditions. The present study constitutes the first 
      attempt to use archived frozen oysters as a source of inoculum, based on the 
      Australian OsHV-1 microVar strain. Experiments were conducted to test (1) virus 
      infectivity, (2) the dose-response relationship for OsHV-1, and (3) the best 
      conditions in which to store infective viral inoculum. Intramuscular injection of 
      a viral inoculum consistently led to an onset of mortality 48 h post-injection 
      and a final cumulative mortality exceeding 90%, in association with high viral 
      loads (1 x 105 to 3 x 107 copies of virus mg-1) in dead individuals. For the 
      first time, an infective inoculum was produced from frozen oysters (tissues 
      stored at -80 degrees C for 6 mo). Storage of purified viral inoculum at +4 degrees C for 3 mo 
      provided similar results to use of fresh inoculum, whereas storage at -20 degrees C, 
      -80 degrees C and room temperature was detrimental to infectivity. A dose-response 
      relationship for OsHV-1 was identified but further research is recommended to 
      determine the most appropriate viral concentration for development of infection 
      models that would be used for different purposes. Overall, this work highlights 
      the best practices and potential issues that may occur in the development of a 
      reproducible and transferable infection model for studying the pathogenicity of 
      the Australian OsHV-1 strain in Crassostrea gigas under experimental conditions.
FAU - Paul-Pont, Ika
AU  - Paul-Pont I
AD  - Faculty of Veterinary Science, The University of Sydney, 425 Werombi Road, 
      Camden, NSW 2570, Australia.
FAU - Evans, Olivia
AU  - Evans O
FAU - Dhand, Navneet K
AU  - Dhand NK
FAU - Whittington, Richard J
AU  - Whittington RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - Australia
MH  - Crassostrea/*virology
MH  - Herpesviridae/*classification/*physiology
MH  - Host-Pathogen Interactions
MH  - Seawater/virology
EDAT- 2015/03/10 06:00
MHDA- 2015/09/26 06:00
CRDT- 2015/03/10 06:00
PHST- 2015/03/10 06:00 [entrez]
PHST- 2015/03/10 06:00 [pubmed]
PHST- 2015/09/26 06:00 [medline]
AID - 10.3354/dao02826 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2015 Mar 9;113(2):137-47. doi: 10.3354/dao02826.