PMID- 25752083
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 9
DP  - 2014 Sep
TI  - [Optimization of sample pretreatment method for the determination of typical 
      artificial sweeteners in soil by high performance liquid chromatography-tandem 
      mass spectrometry].
PG  - 930-5
AB  - The sample pretreatment method for the determination of four typical artificial 
      sweeteners (ASs) including sucralose, saccharin, cyclamate, and acesulfame in 
      soil by high performance liquid chromatography-tandem mass spectrometry 
      (HPLC-MS/MS) was optimized. Different conditions of extraction, including four 
      extractants (methanol, acetonitrile, acetone, deionized water), three kinds of 
      ionic strength of sodium acetate solution (0.001, 0.01, 0.1 mol/L), four pH 
      values (3, 4, 5 and 6) of 0.01 mol/L acetate-sodium acetate solution, four set 
      durations of extraction (20, 40, 60, 120 min) and number of extraction times (1, 
      2, 3, 4 times) were compared. The optimal sample pretreatment method was finally 
      set up. The sam- ples were extracted twice with 25 mL 0.01 mol/L sodium acetate 
      solution (pH 4) for 20 min per cycle. The extracts were combined and then 
      purified and concentrated by CNW Poly-Sery PWAX cartridges with methanol 
      containing 1 mmol/L tris (hydroxymethyl) amino methane (Tris) and 5% (v/v) 
      ammonia hydroxide as eluent. The analytes were determined by HPLC-MS/MS. The 
      recoveries were obtained by spiked soil with the four artificial sweeteners at 1, 
      10, 100 mug/kg (dry weight), separately. The average recoveries of the analytes 
      ranged from 86.5% to 105%. The intra-day and inter-day precisions expressed as 
      relative standard deviations (RSDs) were in the range of 2.56%-5.94% and 
      3.99%-6.53%, respectively. Good linearities (r2 > 0.995) were observed between 
      1-100 mug/kg (dry weight) for all the compounds. The limits of detection were 
      0.01-0.21 kg/kg and the limits of quantification were 0.03-0.70 mug/kg for the 
      analytes. The four artificial sweeteners were determined in soil samples from 
      farmland contaminated by wastewater in Tianjin. This method is rapid, reliable, 
      and suitable for the investigation of artificial sweeteners in soil.
FAU - Feng, Biting
AU  - Feng B
FAU - Gan, Zhiwei
AU  - Gan Z
FAU - Hu, Hongwei
AU  - Hu H
FAU - Sun, Hongwen
AU  - Sun H
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Non-Nutritive Sweeteners)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Non-Nutritive Sweeteners/*analysis
MH  - Soil/*chemistry
MH  - Soil Pollutants/*analysis
MH  - Tandem Mass Spectrometry
EDAT- 2015/03/11 06:00
MHDA- 2015/09/02 06:00
CRDT- 2015/03/11 06:00
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2014.05033 [doi]
PST - ppublish
SO  - Se Pu. 2014 Sep;32(9):930-5. doi: 10.3724/sp.j.1123.2014.05033.