PMID- 25752336
OWN - NLM
STAT- MEDLINE
DCOM- 20160401
LR  - 20181113
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 19
IP  - 5
DP  - 2015 May
TI  - Reduction of protein kinase C alpha (PKC-alpha) promote apoptosis via down-regulation of 
      Dicer in bladder cancer.
PG  - 1085-93
LID - 10.1111/jcmm.12503 [doi]
AB  - In clinic, we examined the expression of protein kinase C (PKC)-alpha and Dicer in 
      the samples of bladder cancer patients, and found that the two proteins have a 
      line correlation. Our study confirmed this correlation existing by clearing the 
      decreasing expression of Dicer after the PKC-alpha knockdown. Treatment of bladder 
      cancer cell lines (T24, 5637) with the PKC-alpha or Dicer knockdown and the PKC 
      inhibitors (Calphostin C and Go 6976) can promote the apoptosis. Inhibition of 
      PKC can increase the activities of caspase-3 and PARP, however, decrease the 
      expression of Dicer. And knockdown of the PKC-alpha or Dicer can also activate the 
      caspase-3 or the PARP. Considering the reduction of PKC-alpha can induce the Dicer 
      down-regulation, we make the conclusion that the reduction of PKC-alpha can promote 
      the apoptosis via the down-regulation of Dicer in bladder cancer.
CI  - (c) 2015 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - Jiang, Zhenming
AU  - Jiang Z
AD  - Department of Urology, The First Affiliated Hospital of China Medical University, 
      Shenyang, China.
FAU - Kong, Chuize
AU  - Kong C
FAU - Zhang, Zhe
AU  - Zhang Z
FAU - Zhu, Yuyan
AU  - Zhu Y
FAU - Zhang, Yuxi
AU  - Zhang Y
FAU - Chen, Xi
AU  - Chen X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150305
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Carbazoles)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Naphthalenes)
RN  - 136194-77-9 (Go 6976)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 2.7.11.13 (PRKCA protein, human)
RN  - EC 2.7.11.13 (Protein Kinase C-alpha)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - EC 3.4.22.- (Caspase 3)
RN  - I271P23G24 (calphostin C)
SB  - IM
MH  - Apoptosis/drug effects/*genetics
MH  - Blotting, Western
MH  - Carbazoles/pharmacology
MH  - Caspase 3/metabolism
MH  - Cell Line, Tumor
MH  - *Down-Regulation
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Naphthalenes/pharmacology
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Protein Kinase C-alpha/antagonists & inhibitors/*genetics/metabolism
MH  - *RNA Interference
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribonuclease III/*genetics/metabolism
MH  - Urinary Bladder Neoplasms/genetics/metabolism/pathology
PMC - PMC4420610
OTO - NOTNLM
OT  - Dicer
OT  - PKC-alpha
OT  - apoptosis
OT  - bladder cancer
OT  - caspase-3
EDAT- 2015/03/11 06:00
MHDA- 2016/04/02 06:00
PMCR- 2015/05/01
CRDT- 2015/03/11 06:00
PHST- 2014/05/15 00:00 [received]
PHST- 2014/10/28 00:00 [accepted]
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2016/04/02 06:00 [medline]
PHST- 2015/05/01 00:00 [pmc-release]
AID - 10.1111/jcmm.12503 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2015 May;19(5):1085-93. doi: 10.1111/jcmm.12503. Epub 2015 Mar 5.