PMID- 25752697
OWN - NLM
STAT- MEDLINE
DCOM- 20150824
LR  - 20181202
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 135
DP  - 2015 Jun
TI  - Morphology and neurochemistry of rabbit iris innervation.
PG  - 182-91
LID - S0014-4835(15)00087-1 [pii]
LID - 10.1016/j.exer.2015.03.005 [doi]
AB  - The aim of this study was to map the entire nerve architecture and sensory 
      neuropeptide content of the rabbit iris. Irises from New Zealand rabbits were 
      stained with antibodies against neuronal-class betaIII-tubulin, calcitonin 
      gene-related peptide (CGRP) and substance P (SP), and whole-mount images were 
      acquired to build a two-dimensional view of the iridal nerve architecture. After 
      taking images in time-lapse mode, we observed thick nerves running in the iris 
      stroma close to the anterior epithelia, forming four to five stromal nerve rings 
      from the iris periphery to the pupillary margin and sub-branches that connected 
      with each other, constituting the stromal nerve plexus. In the anterior side, 
      fine divisions derivated from the stromal nerves, forming a nerve network-like 
      structure to innervate the superficial anterior border layer, with the pupillary 
      margin having the densest innervation. In the posterior side, the nerve bundles 
      ran along with the pupil dilator muscle in a radial pattern. The morphology of 
      the iris nerves on both sides changed with pupil size. To obtain the relative 
      content of the neuropeptides in the iris, the specimens were double stained with 
      betaIII-tubulin and CGRP or SP antibodies. Relative nerve fiber densities for each 
      fiber population were assessed quantitatively by computer-assisted analysis. On 
      the anterior side, CGRP-positive nerve fibers constituted about 61%, while 
      SP-positive nerves constitute about 30.5%, of the total nerve content, which was 
      expressed as betaIII tubulin-positive fibers. In addition, in the anterior stroma of 
      the collarette region, there were non-neuronal cells that were positive for SP. 
      On the posterior side, CGRP-positive nerve fibers were about 69% of total nerve 
      content, while SP constituted only up to 20%. Similarly, in the trigeminal 
      ganglia (TG), the number of CGRP-positive neurons significantly outnumbered those 
      that were positive for SP. Also, all the SP-positive neurons were labeled with 
      CGRP. This is the first study to provide a two-dimensional whole mount and a 
      cross-sectional view of the entire iris nerve architecture. Considering the 
      anatomical location, the high expression of CGRP and SP suggests that these 
      neuropeptides may play a role in the pathogenesis of anterior uveitis, glaucoma, 
      cataracts and chronic ocular pain.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - He, Jiucheng
AU  - He J
AD  - Neuroscience Center of Excellence and the Department of Ophthalmology, Louisiana 
      State University Health Sciences Center, School of Medicine, New Orleans, LA, 
      USA. Electronic address: jhe@lsuhsc.edu.
FAU - Bazan, Haydee E P
AU  - Bazan HE
AD  - Neuroscience Center of Excellence and the Department of Ophthalmology, Louisiana 
      State University Health Sciences Center, School of Medicine, New Orleans, LA, 
      USA. Electronic address: hbazanl@lsuhsc.edu.
LA  - eng
GR  - EY019465/EY/NEI NIH HHS/United States
GR  - 149750141G/PHS HHS/United States
GR  - GM103340/GM/NIGMS NIH HHS/United States
GR  - R01 EY019465/EY/NEI NIH HHS/United States
GR  - P30 GM103340/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150307
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Neuropeptides)
RN  - 33507-63-0 (Substance P)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Animals
MH  - Calcitonin Gene-Related Peptide/analysis
MH  - Iris/*innervation
MH  - Nerve Fibers/*chemistry
MH  - Neuropeptides/*analysis
MH  - Pupil/physiology
MH  - Rabbits/*anatomy & histology
MH  - Substance P/analysis
MH  - Trigeminal Ganglion/cytology
PMC - PMC4741101
MID - NIHMS676832
OTO - NOTNLM
OT  - Anterior uveitis
OT  - Calcitonin gene-related peptide
OT  - Iris innervation
OT  - Neurogenic inflammation
OT  - Neuropeptides
OT  - Sensory nerves
OT  - Substance P
OT  - Trigeminal ganglia
EDAT- 2015/03/11 06:00
MHDA- 2015/08/25 06:00
PMCR- 2016/06/01
CRDT- 2015/03/11 06:00
PHST- 2014/06/11 00:00 [received]
PHST- 2015/02/12 00:00 [revised]
PHST- 2015/03/05 00:00 [accepted]
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2015/08/25 06:00 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - S0014-4835(15)00087-1 [pii]
AID - 10.1016/j.exer.2015.03.005 [doi]
PST - ppublish
SO  - Exp Eye Res. 2015 Jun;135:182-91. doi: 10.1016/j.exer.2015.03.005. Epub 2015 Mar 
      7.