PMID- 25752819
OWN - NLM
STAT- MEDLINE
DCOM- 20160510
LR  - 20181202
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 116
IP  - 9
DP  - 2015 Sep
TI  - Protein Kinase C Is Involved in the Induction of ATP-Binding Cassette Transporter 
      A1 Expression by Liver X Receptor/Retinoid X Receptor Agonist in Human 
      Macrophages.
PG  - 2032-8
LID - 10.1002/jcb.25157 [doi]
AB  - The transcription of the ATP-binding cassette transporter A1 (ABCA1) gene, which 
      plays a key anti-atherogenic role, is known to be induced by agonists of liver X 
      receptors (LXRs). LXRs form obligate heterodimers with retinoid X receptors 
      (RXRs) and interact with their recognition sequences in the regulatory regions of 
      key genes implicated in the control of cholesterol, fatty acid and glucose 
      homeostasis. We have previously shown a novel role for c-Jun N-terminal kinase 
      (JNK) and phosphoinositide 3-kinase (PI3K) in the LXRs-mediated induction of 
      macrophage gene expression. Protein kinase C (PKC) is often found to regulate the 
      action of nuclear receptors and cross talk between this kinase family and JNK 
      and/or PI3K has been shown in several settings. We have, therefore, investigated 
      a potential role for PKC in the action of LXR/RXR agonist 
      22-(R)-hydroxycholesterol (22-(R)-HC)/9-cis-retinoic acid (9cRA) in THP-1 
      macrophages, including the induction of ABCA1 expression. The pan PKC inhibitor 
      bisindoylmaleimide was found to attenuate the induction of ABCA1 protein 
      expression, the activation of the JNK signaling pathway and the stimulation of 
      activator protein-1 (AP-1) DNA binding activity in macrophages treated with 
      22-(R)-HC and 9cRA. The role of PKC in the action of these ligands was confirmed 
      further by the use of more isotype-specific inhibitors. These studies, therefore, 
      reveal a potentially important role for PKC in the action of 22-(R)-HC and 9cRA 
      in human macrophages.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Huwait, Etimad A
AU  - Huwait EA
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Singh, Nishi N
AU  - Singh NN
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Michael, Daryn R
AU  - Michael DR
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Davies, Thomas S
AU  - Davies TS
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Moss, Joe W E
AU  - Moss JW
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
FAU - Ramji, Dipak P
AU  - Ramji DP
AD  - Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, 
      Museum Avenue, Cardiff, CF10 3AX, United Kingdom.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (ABCA1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (Hydroxycholesterols)
RN  - 0 (Indoles)
RN  - 0 (Liver X Receptors)
RN  - 0 (Maleimides)
RN  - 0 (Orphan Nuclear Receptors)
RN  - 0 (Retinoid X Receptors)
RN  - 17711-16-9 (22-hydroxycholesterol)
RN  - 1UA8E65KDZ (Alitretinoin)
RN  - 5688UTC01R (Tretinoin)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - L79H6N0V6C (bisindolylmaleimide I)
SB  - IM
MH  - ATP Binding Cassette Transporter 1/antagonists & inhibitors/*metabolism
MH  - Alitretinoin
MH  - Cell Line
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Hydroxycholesterols/*pharmacology
MH  - Indoles/pharmacology
MH  - Liver X Receptors
MH  - MAP Kinase Signaling System/drug effects
MH  - Macrophages/cytology/*drug effects
MH  - Maleimides/pharmacology
MH  - Orphan Nuclear Receptors/agonists/metabolism
MH  - Protein Kinase C/*metabolism
MH  - Retinoid X Receptors/agonists/metabolism
MH  - Tretinoin/*pharmacology
OTO - NOTNLM
OT  - ATHEROSCLEROSIS
OT  - CELL SIGNALING
OT  - GENE EXPRESSION
OT  - LIVER X RECEPTORS
OT  - MACROPHAGES
OT  - NUCLEAR RECEPTORS
EDAT- 2015/03/11 06:00
MHDA- 2016/05/11 06:00
CRDT- 2015/03/11 06:00
PHST- 2014/07/03 00:00 [received]
PHST- 2015/03/03 00:00 [accepted]
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2016/05/11 06:00 [medline]
AID - 10.1002/jcb.25157 [doi]
PST - ppublish
SO  - J Cell Biochem. 2015 Sep;116(9):2032-8. doi: 10.1002/jcb.25157.