PMID- 25753408
OWN - NLM
STAT- MEDLINE
DCOM- 20160104
LR  - 20150408
IS  - 1872-7549 (Electronic)
IS  - 0166-4328 (Linking)
VI  - 286
DP  - 2015 Jun 1
TI  - Maternal vitamin D deficiency alters fetal brain development in the BALB/c mouse.
PG  - 192-200
LID - S0166-4328(15)00152-7 [pii]
LID - 10.1016/j.bbr.2015.03.008 [doi]
AB  - Prenatal exposure to vitamin D is thought to be critical for optimal fetal 
      neurodevelopment, yet vitamin D deficiency is apparent in a growing proportion of 
      pregnant women. The aim of this study was to determine whether a mouse model of 
      vitamin D-deficiency alters fetal neurodevelopment. Female BALB/c mice were 
      placed on either a vitamin D control (2,195 IU/kg) or deficient (0 IU/kg) diet 
      for 5 weeks prior to and during pregnancy. Fetal brains were collected at 
      embryonic day (E) 14.5 or E17.5 for morphological and gene expression analysis. 
      Vitamin D deficiency during pregnancy reduced fetal crown-rump length and head 
      size. Moreover, lateral ventricle volume was reduced in vitamin D-deficient 
      foetuses. Expression of neurotrophin genes brain-derived neurotrophic factor 
      (Bdnf) and transforming growth factor-beta1 (Tgf-beta1) was altered, with Bdnf reduced 
      at E14.5 and increased at E17.5 following vitamin D deficiency. Brain expression 
      of forkhead box protein P2 (Foxp2), a gene known to be important in human speech 
      and language, was also altered. Importantly, Foxp2 immunoreactive cells in the 
      developing cortex were reduced in vitamin D-deficient female foetuses. At E17.5, 
      brain tyrosine hydroxylase (TH) gene expression was reduced in females, as was TH 
      protein localization (to identify dopamine neurons) in the substantia nigra of 
      vitamin D-deficient female foetuses. Overall, we show that prenatal vitamin 
      D-deficiency leads to alterations in fetal mouse brain morphology and genes 
      related to neuronal survival, speech and language development, and dopamine 
      synthesis. Vitamin D appears to play an important role in mouse neurodevelopment.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Hawes, Jazmin E
AU  - Hawes JE
AD  - School of Anatomy, Physiology and Human Biology, The University of Western 
      Australia, Perth 6009, Australia.
FAU - Tesic, Dijana
AU  - Tesic D
AD  - School of Anatomy, Physiology and Human Biology, The University of Western 
      Australia, Perth 6009, Australia.
FAU - Whitehouse, Andrew J
AU  - Whitehouse AJ
AD  - Telethon Kids Institute, The University of Western Australia, Perth 6009, 
      Australia.
FAU - Zosky, Graeme R
AU  - Zosky GR
AD  - School of Medicine, University of Tasmania, Hobart 7000, Australia.
FAU - Smith, Jeremy T
AU  - Smith JT
AD  - School of Anatomy, Physiology and Human Biology, The University of Western 
      Australia, Perth 6009, Australia.
FAU - Wyrwoll, Caitlin S
AU  - Wyrwoll CS
AD  - School of Anatomy, Physiology and Human Biology, The University of Western 
      Australia, Perth 6009, Australia. Electronic address: caitlin.wyrwoll@uwa.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150307
PL  - Netherlands
TA  - Behav Brain Res
JT  - Behavioural brain research
JID - 8004872
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp2 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - Brain/*embryology/*metabolism/pathology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Crown-Rump Length
MH  - Disease Models, Animal
MH  - Dopaminergic Neurons/metabolism/pathology
MH  - Female
MH  - Forkhead Transcription Factors/metabolism
MH  - Head/embryology
MH  - Immunohistochemistry
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Pregnancy
MH  - Pregnancy Complications/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Repressor Proteins/metabolism
MH  - Sex Characteristics
MH  - Transforming Growth Factor beta1/metabolism
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - Vitamin D Deficiency/*metabolism
OTO - NOTNLM
OT  - Foxp2
OT  - Neurodevelopment
OT  - Tyrosine hydroxylase
OT  - Vitamin D
EDAT- 2015/03/11 06:00
MHDA- 2016/01/05 06:00
CRDT- 2015/03/11 06:00
PHST- 2015/01/28 00:00 [received]
PHST- 2015/03/02 00:00 [revised]
PHST- 2015/03/02 00:00 [accepted]
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2016/01/05 06:00 [medline]
AID - S0166-4328(15)00152-7 [pii]
AID - 10.1016/j.bbr.2015.03.008 [doi]
PST - ppublish
SO  - Behav Brain Res. 2015 Jun 1;286:192-200. doi: 10.1016/j.bbr.2015.03.008. Epub 
      2015 Mar 7.