PMID- 25753465
OWN - NLM
STAT- MEDLINE
DCOM- 20160614
LR  - 20221207
IS  - 1099-0801 (Electronic)
IS  - 0269-3879 (Linking)
VI  - 29
IP  - 10
DP  - 2015 Oct
TI  - Simple extraction method using syringe filter for detection of ethephon in 
      tomatoes by negative-ion mode liquid chromatography with tandem mass 
      spectrometry.
PG  - 1480-5
LID - 10.1002/bmc.3446 [doi]
AB  - In this study, a simple, rapid, and sensitive method was developed for the 
      extraction of ethephon from homogenized tomatoes that does not require a cleanup 
      procedure. In a syringe filter, three distinct layers - aqueous, acetonitrile, 
      and n-hexane - are clearly separated after storage at -80  degrees C for 5-10 min. A 
      Dionex IonPac column was used to separate the analyte before detection using 
      negative-ion mode liquid chromatography with tandem mass spectrometry (LC/MS/MS). 
      The matrix effect of the tested analyte was negligibly small and the matched 
      calibration showed a good linearity over a concentration range of 0.01-1.0 mg/kg 
      with a correlation coefficient (R(2) ) of 0.9998. The recovery at three 
      fortification levels (0.1, 0.5 and 1.0 mg/kg) was between 82.9 and 108.6% with 
      relative standard deviations (RSDs) <5.0%. The limit of quantification 
      (0.03 mg/kg) was lower than the maximum residue limit (3 mg/kg) set by the 
      Ministry of Food and Drug Safety, Republic of Korea. From a field trial, the 
      method developed herein was applied to calculate the decline pattern and predict 
      the pre-harvest residue limits of ethephon in tomatoes. In conclusion, the 
      proposed sample preparation is feasible for the detection of hydrophilic analytes 
      in tomatoes.
CI  - Copyright (c) 2015 John Wiley & Sons, Ltd.
FAU - Cho, Soon-Kil
AU  - Cho SK
AD  - National Agricultural Products Quality Management Service, Products Safety 
      Inspection Laboratory, Gwangsan-Gu, Gwangju, Republic of Korea.
FAU - Cho, Ji-Mi
AU  - Cho JM
AD  - National Agricultural Products Quality Management Service, Products Safety 
      Inspection Laboratory, Gwangsan-Gu, Gwangju, Republic of Korea.
FAU - Abd El-Aty, A M
AU  - Abd El-Aty AM
AD  - Biotechnology Research Institute, College of Agriculture and Life Sciences, 
      Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, 
      Republic of Korea.
AD  - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 
      Giza, 12211, Egypt.
AD  - Department of Veterinary Pharmacology and Toxicology, College of Veterinary 
      Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul, 143-701, 
      Republic of Korea.
FAU - Rahman, Md Musfiqur
AU  - Rahman MM
AD  - Biotechnology Research Institute, College of Agriculture and Life Sciences, 
      Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, 
      Republic of Korea.
FAU - Choi, Jeong-Heui
AU  - Choi JH
AD  - Biotechnology Research Institute, College of Agriculture and Life Sciences, 
      Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, 
      Republic of Korea.
FAU - Seo, Young-Jun
AU  - Seo YJ
AD  - National Agricultural Products Quality Management Service, Products Safety 
      Inspection Laboratory, Gwangsan-Gu, Gwangju, Republic of Korea.
FAU - Shin, Ho-Chul
AU  - Shin HC
AD  - Department of Veterinary Pharmacology and Toxicology, College of Veterinary 
      Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul, 143-701, 
      Republic of Korea.
FAU - Shim, Jae-Han
AU  - Shim JH
AD  - Biotechnology Research Institute, College of Agriculture and Life Sciences, 
      Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, 
      Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20150304
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Organophosphorus Compounds)
RN  - XU5R5VQ87S (ethephon)
SB  - IM
MH  - Calibration
MH  - Chemical Fractionation/instrumentation/*methods
MH  - Chromatography, Liquid/*methods
MH  - Equipment Design
MH  - Food Analysis/methods
MH  - Half-Life
MH  - Limit of Detection
MH  - Solanum lycopersicum/*chemistry/drug effects
MH  - Organophosphorus Compounds/*analysis/pharmacology
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - analysis
OT  - extraction
OT  - method validation
OT  - plant growth regulator
OT  - residue
OT  - tandem mass spectrometry
OT  - tomato
EDAT- 2015/03/11 06:00
MHDA- 2016/06/15 06:00
CRDT- 2015/03/11 06:00
PHST- 2014/12/01 00:00 [received]
PHST- 2015/01/06 00:00 [revised]
PHST- 2015/01/15 00:00 [accepted]
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2016/06/15 06:00 [medline]
AID - 10.1002/bmc.3446 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2015 Oct;29(10):1480-5. doi: 10.1002/bmc.3446. Epub 2015 Mar 
      4.