PMID- 25754332
OWN - NLM
STAT- MEDLINE
DCOM- 20160222
LR  - 20240518
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 19
IP  - 8
DP  - 2015 Aug
TI  - Botulinum neurotoxin type A modulates vesicular release of glutamate from 
      satellite glial cells.
PG  - 1900-9
LID - 10.1111/jcmm.12562 [doi]
AB  - This study investigated the presence of cell membrane docking proteins 
      synaptosomal-associated protein, 25 and 23 kD (SNAP-25 and SNAP-23) in satellite 
      glial cells (SGCs) of rat trigeminal ganglion; whether cultured SGCs would 
      release glutamate in a time- and calcium-dependent manner following 
      calcium-ionophore ionomycin stimulation; and if botulinum neurotoxin type A 
      (BoNTA), in a dose-dependent manner, could block or decrease vesicular release of 
      glutamate. SGCs were isolated from the trigeminal ganglia (TG) of adult Wistar 
      rats and cultured for 7 days. The presence of SNAPs in TG sections and isolated 
      SGCs were investigated using immunohistochemistry and immunocytochemistry, 
      respectively. SGCs were stimulated with ionomycin (5 muM for 4, 8, 12 and 30 min.) 
      to release glutamate. SGCs were then pre-incubated with BoNTA (24 hrs with 0.1, 
      1, 10 and 100 pM) to investigate if BoNTA could potentially block 
      ionomycin-stimulated glutamate release. Glutamate concentrations were measured by 
      ELISA. SNAP-25 and SNAP-23 were present in SGCs in TG sections and in cultured 
      SGCs. Ionomycin significantly increased glutamate release from cultured SGCs 30 
      min. following the treatment (P < 0.001). BoNTA (100 pM) significantly decreased 
      glutamate release (P < 0.01). Results from this study demonstrated that SGCs, 
      when stimulated with ionomycin, released glutamate that was inhibited by BoNTA, 
      possibly through cleavage of SNAP-25 and/or SNAP-23. These novel findings 
      demonstrate the existence of vesicular glutamate release from SGCs, which could 
      potentially play a role in the trigeminal sensory transmission. In addition, 
      interaction of BoNTA with non-neuronal cells at the level of TG suggests a 
      potential analgesic mechanism of action of BoNTA.
CI  - (c) 2015 The Authors. Journal of Cellular and Molecular Medicine published by John 
      Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
FAU - da Silva, Larissa Bittencourt
AU  - da Silva LB
AD  - Center for Sensory - Motor Interaction (SMI), Department of Health Science and 
      Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.
FAU - Poulsen, Jeppe Norgaard
AU  - Poulsen JN
AD  - Center for Sensory - Motor Interaction (SMI), Department of Health Science and 
      Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.
FAU - Arendt-Nielsen, Lars
AU  - Arendt-Nielsen L
AD  - Center for Sensory - Motor Interaction (SMI), Department of Health Science and 
      Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.
FAU - Gazerani, Parisa
AU  - Gazerani P
AD  - Center for Sensory - Motor Interaction (SMI), Department of Health Science and 
      Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.
AD  - Laboratory for Cancer Biology, Biomedicine, Department of Health Science and 
      Technology, Faculty of Medicine, Aalborg University, Aalborg East, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150305
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Snap23 protein, rat)
RN  - 0 (Synaptosomal-Associated Protein 25)
RN  - 0 (Vesicular Transport Proteins)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 56092-81-0 (Ionomycin)
RN  - EC 3.4.24.69 (Botulinum Toxins, Type A)
SB  - IM
MH  - Animals
MH  - Botulinum Toxins, Type A/*pharmacology
MH  - Cells, Cultured
MH  - Glutamic Acid/*metabolism
MH  - Immunohistochemistry
MH  - Ionomycin/pharmacology
MH  - Male
MH  - Neuroglia/drug effects/*metabolism
MH  - Rats, Wistar
MH  - Synaptic Vesicles/drug effects/*metabolism
MH  - Synaptosomal-Associated Protein 25/metabolism
MH  - Time Factors
MH  - Trigeminal Ganglion/cytology/metabolism
MH  - Vesicular Transport Proteins/metabolism
PMC - PMC4549040
OTO - NOTNLM
OT  - SNAP-23
OT  - SNAP-25
OT  - botulinum neurotoxin type A
OT  - glutamate
OT  - ionomycin
OT  - pain
OT  - satellite glial cells
OT  - trigeminal ganglion
OT  - vesicular release
EDAT- 2015/03/11 06:00
MHDA- 2016/02/24 06:00
PMCR- 2015/08/01
CRDT- 2015/03/11 06:00
PHST- 2014/05/11 00:00 [received]
PHST- 2015/01/20 00:00 [accepted]
PHST- 2015/03/11 06:00 [entrez]
PHST- 2015/03/11 06:00 [pubmed]
PHST- 2016/02/24 06:00 [medline]
PHST- 2015/08/01 00:00 [pmc-release]
AID - 10.1111/jcmm.12562 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2015 Aug;19(8):1900-9. doi: 10.1111/jcmm.12562. Epub 2015 Mar 5.